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Product Info Summary
| SKU: | M03877 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HOMER1 Mouse Monoclonal Antibody [Clone ID: OTI7G10]
SKU/Catalog Number
M03877
Size
100 µl
Description
Boster Bio HOMER1 mouse monoclonal antibody,clone OTI7G10. Catalog# M03877. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-HOMER1 Mouse Monoclonal Antibody [Clone ID: OTI7G10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03877)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI7G10
Isotype
IgG2b
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-354 of human HOMER1 (NP_004263) produced in E.coli.
Reactive Species
M03877 is reactive to HOMER1 in Human, Mouse, Rat
Observed Molecular Weight
46,50 kDa
Calculated molecular weight
40.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03877 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
IHC: 1:500
Positive Control
WB:rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of HOMER1 using anti-HOMER1 antibody (M03877).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HOMER1 antigen affinity purified monoclonal antibody (M03877) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HOMER1 at approximately 46,50 kDa. The expected band size for HOMER1 is at 40 kDa.
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Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-HOMER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-HOMER1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Specific Publications For Anti-HOMER1 Mouse Monoclonal Antibody [Clone ID: OTI7G10] (M03877)
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