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Product Info Summary
| SKU: | A00972-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-Follistatin/FST Antibody Picoband®
SKU/Catalog Number
A00972-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Follistatin/FST Antibody Picoband® catalog # A00972-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Follistatin/FST Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00972-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Follistatin recombinant protein (Position: N31-K261).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00972-1 is reactive to FST in Human, Mouse, Rat
Observed Molecular Weight
40 kDa
Calculated molecular weight
38.0 kDa
Background of FST
Follistatin also known as activin-binding protein is a protein that in humans is encoded by the FST gene. Follistatin is an autocrine glycoprotein that is expressed in nearly all tissues of higher animals. It is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00972-1 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: mouse kidney tissue, human MCF-7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Follistatin using anti-Follistatin antibody (A00972-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse kidney tissue lysates,
Lane 2: human MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Follistatin antigen affinity purified polyclonal antibody (Catalog # A00972-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Follistatin at approximately 40KD. The expected band size for Follistatin is at 38KD.
Specific Publications For Anti-Follistatin/FST Antibody Picoband® (A00972-1)
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1 Customer Q&As for Anti-Follistatin/FST Antibody Picoband®
Question
We are currently using anti-Follistatin/FST antibody A00972-1 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
J. Wu
Verified customer
Asked: 2018-12-25
Answer
The anti-Follistatin/FST antibody (A00972-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-25
