Product Info Summary
| SKU: | PB9216 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HOXA9 Antibody Picoband®
SKU/Catalog Number
PB9216
PB0214 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HOXA9 Antibody Picoband® catalog # PB9216. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HOXA9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9216)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HOXA9 recombinant protein (Position: M1-R204). Human HOXA9 shares 98% amino acid (aa) sequence identity with mouse HOXA9.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9216 is reactive to HOXA9 in Human, Rat
Observed Molecular Weight
30 kDa
Calculated molecular weight
30.2 kDa
Background of HOXA9
Homeobox protein Hox-A9 is a protein that in humans is encoded by the HOXA9 gene. It is mapped to 7p15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA9 is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. As HOXA9 is part of the homeobox family, involved in setting the body plans of animals, it is likely that HOXA9 would display increased expression in cells with higher differentiation potentials.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9216 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Testis Tissue, HEPG2 Whole Cell, HEPA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-HOXA9 antibody, PB9216, Western blotting
All lanes: Anti HOXA9 (PB9216) at 0.5ug/ml
WB: Recombinant Human HOXA9 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Click image to see more details
Anti-HOXA9 antibody, PB9216, Western blotting
All lanes: Anti HOXA9 (PB9216) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 30KD
Observed bind size: 30KD
Specific Publications For Anti-HOXA9 Antibody Picoband® (PB9216)
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Customer Q&As
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4 Customer Q&As for Anti-HOXA9 Antibody Picoband®
Question
We have seen staining in rat colon. Are there any suggestions? Is anti-HOXA9 antibody supposed to stain colon positively?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
From literature colon does express HOXA9. From Uniprot.org, HOXA9 is expressed in metanephros, bone marrow, colon, among other tissues. Regarding which tissues have HOXA9 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 9880515
Colon, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-19
Question
We are currently using anti-HOXA9 antibody PB9216 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The anti-HOXA9 antibody (PB9216) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-29
Question
Our lab were well pleased with the WB result of your anti-HOXA9 antibody. However we have observed positive staining in metanephros nucleus. using this antibody. Is that expected? Could you tell me where is HOXA9 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
Based on literature, metanephros does express HOXA9. Generally HOXA9 expresses in nucleus. Regarding which tissues have HOXA9 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 9880515
Colon, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-04-05
Question
I was wanting to use using your anti-HOXA9 antibody for male gonad development studies. Has this antibody been tested with western blotting on rat testis tissue? We would like to see some validation images before ordering.
R. Roberts
Verified customer
Asked: 2015-07-31
Answer
We appreciate your inquiry. This PB9216 anti-HOXA9 antibody is tested on rat testis tissue, tissue lysate, hepa whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-07-31


