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Product Info Summary
| SKU: | A09541 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-FXYD6 Antibody (C-term)
SKU/Catalog Number
A09541
Size
80 µl
Description
Boster Bio Anti-FXYD6 Antibody (C-term) (Catalog # A09541). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-FXYD6 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09541)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This FXYD6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 81-115amino acids from the C-terminal region of human FXYD6.
Reactive Species
A09541 is reactive to FXYD6 in Human, Mouse, Rat
Calculated molecular weight
10.5 kDa
Background of FXYD6
THDBP1 is a nuclear transcription factor involved in the activation of the solute carrier family 2 member 4 gene. This protein interacts with another transcription factor, myocyte enhancer factor 2, to activate transcription of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09541 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-FXYD6 Antibody (C-term) at 1:1000 dilution
Lane 1: Human cerebellum tissue lysate
Lane 2: Human brain tissue lysate
Lane 3: SH-SY5Y whole cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse cerebellum tissue lysate
Lane 6: Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 11 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded H. liver section using FXYD6 Antibody (C-term). A09541 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded M. kidney section using FXYD6 Antibody (C-term). A09541 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of SH-SY5Y cells using FXYD6 Antibody (C-term)(green, Cat#A09541) compared to an isotype control of rabbit IgG(blue). A09541 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-FXYD6 Antibody (C-term) (A09541)
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