Mouse IL7R/CD127 ELISA Kit PicoKine®

Product Name

Mouse IL7R/CD127 ELISA Kit PicoKine®

SKU/Catalog Number

EK0714

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL7R/CD127 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il7r in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IL7R/CD127 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0714)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: E21-D239

Capture/Detection Antibodies

The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat.

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0714 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0714 is reactive to Il7r in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0714 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Il7r

The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

Kit Components

Catalog Number	Description	Quantity
EK0714-CAP	Anti-Mouse Il7r Pre-coated 96-well strip microplate	1
EK0714-ST	Mouse Il7r Standard	2 vials, 20 ng/tube
EK0714-DA	Mouse Il7r Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.002	0.031	0.058	0.117	0.266	0.531	1.107	2.085

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Il7r, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 31.2 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL7R/CD127 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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