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Product Info Summary
| SKU: | A00676S15 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-HSP27 (phospho-S15) HSPB1 Antibody
SKU/Catalog Number
A00676S15
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HSP27 (phospho-S15) HSPB1 Antibody catalog # A00676S15. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP27 (phospho-S15) HSPB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00676S15)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Serine 15.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00676S15 is reactive to HSPB1 in Human, Mouse, Rat
Calculated molecular weight
22.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00676S15 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IP: 1:10-1:100
Validation Images & Assay Conditions
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Western blot (WB) analysis of HSP27 (phospho-S15) pAb at 1:500 dilution Lane1:L02 whole cell lysate Lane2:SGC7901 whole cell lysate Lane3:HEK293T transfected with pCDNA3.1-Flag-HSP27 whole cell lysate Lane4:HEK293T whole cell lysate
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Immunoprecipitation of L02 whole cell lysate using HSP27 (phospho-S15) pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4) .Lane 1 is 20% input. The western blot was probed using HSP27 (phospho-S15) pAb #AP0245.“↑”（supernatant）; “↓”（deposition）
Specific Publications For Anti-HSP27 (phospho-S15) HSPB1 Antibody (A00676S15)
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