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- Table of Contents
HSP90AB1 antibody (M01692-4) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of CACO-2 cells using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4).
Overlay histogram showing CACO-2 cells stained with M01692-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with M01692-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5)
HSP90AB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
HSP90AB1 antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human Raji whole cell, human A431 whole cell, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HSP90AB1 antibody was positively detected by IHC in:
human thyroid cancer tissue, human placenta tissue, human laryngeal squamous cell carcinoma tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HSP90AB1 antibody was positively detected by ICC/IF in:
MCF-7 cell
HSP90AB1 antibody was positively detected by FC in:
CACO-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 µg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 µg/ml
Human
Flow Cytometry (Fixed)
1-3 µg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5)
View all HSP90AB1 Antibodies
View all HSP90AB1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
7B7F5
Isotype
IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp90 beta, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
90 kDa
Calculated Molecular Weight
83.3 kDa
Background of HSP90AB1
Heat shock protein HSP 90-beta, also called HSP90beta, is a protein that in humans is encoded by the HSP90AB1 gene. It is mapped to chromosome 6p21.1. This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. And this gene is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.
Cite This Product
Anti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01692-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
82%
Moderate Homology
Drosophila
58%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HSP90AB1 antibody
Immunohistochemistry (IHC) protocol for HSP90AB1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HSP90AB1 antibody
Flow Cytometry (FC) protocol for HSP90AB1 antibody
Publications
Application:
Species:
Single-cell RNA sequencing of estrual mice reveals PM2.5-induced uterine cell heterogeneity and reproductive toxicity
Published: 2024-09-04
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: IF
Impact Factor: 6.20
Species: Mouse
PMID: 39236655
Review
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Product Promise
We promise Anti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01692-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026