Anti-Hsp90 beta/HSP90AB1 Antibody Picoband™ (monoclonal, 7B7F5)

Product Name

Anti-Hsp90 beta/HSP90AB1 Antibody Picoband™ (monoclonal, 7B7F5)

View all HSP90 beta Antibodies

SKU/Catalog Number

M01692-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hsp90 beta/HSP90AB1 Antibody Picoband™ (monoclonal, 7B7F5) catalog # M01692-4. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Hsp90 beta/HSP90AB1 Antibody Picoband™ (monoclonal, 7B7F5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01692-4)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

7B7F5

Isotype

IgG2b

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp90 beta, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01692-4 is reactive to HSP90AB1 in Human, Mouse, Rat

Applications

M01692-4 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

90 kDa

Calculated molecular weight

83.264kDa

Background of HSP90 beta

Heat shock protein HSP 90-beta, also called HSP90beta, is a protein that in humans is encoded by the HSP90AB1 gene. It is mapped to chromosome 6p21.1. This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. And this gene is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Hsp90 beta/HSP90AB1 using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Hsp90 beta/HSP90AB1 antigen affinity purified monoclonal antibody (Catalog # M01692-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Hsp90 beta/HSP90AB1 at approximately 90 kDa. The expected band size for Hsp90 beta/HSP90AB1 is at 84 kDa.

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Figure 2. IF analysis of Hsp90 beta/HSP90AB1 using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4).
Hsp90 beta/HSP90AB1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4).
Overlay histogram showing CACO-2 cells stained with M01692-4 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For HSP90AB1 (Source: Uniprot.org, NCBI)

Alternative Names

D6S182; Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein beta; heat shock protein HSP 90-beta; HSP 84; HSP84; HSP90 beta; HSP90AB1; HSP90B; HSP90-BETA; HSP90BHSP 90; HSPC2; HSPC2D6S182; HSPCB; HSPCBFLJ26984 HSP90AB1 D6S182, HSP84, HSP90B, HSPC2, HSPCB heat shock protein 90 alpha family class B member 1 heat shock protein HSP 90-beta|HSP90-beta|heat shock 84 kDa|heat shock 90kD protein 1, beta|heat shock protein 90 kDa|heat shock protein 90kDa alpha (cytosolic), class B member 1|heat shock protein 90kDa alpha family class B member 1

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In this infographic, you will see the following information for HSP90AB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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