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Pack Size:100μg/vial
Validated Species:Chicken, Human, Mouse
Clone Number:FN-15
Application:IHC, ICC, WB
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Product Name Anti-Fibronectin Monoclonal Antibody
SKU/Catalog Number MA1116
Description Mouse IgG monoclonal antibody for Fibronectin, fibronectin 1 (FN1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Fibronectin Monoclonal Antibody FN-15 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1116)
Replacement Item This antibody may replace the following items: sc-18825|sc-18826|sc-18827|sc-271098|sc-50078|sc-50079|sc-50080|sc-50081|sc-52331|sc-53285|sc-53286|sc-59824|sc-59826|sc-6952|sc-6953|sc-69681|sc-69682|sc-73611|sc-81767|sc-81769|sc-8422|sc-9068|sc-98454 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken, Human, Mouse
Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Fibronectin from human plasma.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Monoclonal
Clone Number FN-15
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Fn1
Protein Name Fibronectin
Molecular Weight 272511 MW
Protein Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). .
Tissue Specificity Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
Sequence Similarities Contains 12 fibronectin type-I domains.
Subcellular Localization Secreted, extracellular space, extracellular matrix.
Uniprot ID P04937
Alternative Names Fibronectin;FN;Anastellin;Fn1;
Research Areas FN1|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Fibronectin

Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to fibronectin 1 antibody, fn1 antibody, msf antibody