Anti-Fibronectin Fn1 Monoclonal Antibody

Boster Bio Anti-Fibronectin Fn1 Monoclonal Antibody catalog # MA1116. Tested in IHC, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse. Cited in 25 publication(s).

Product Info Summary

SKU: MA1116
Size: 100μg/vial
Reactive Species: Chicken, Human, Mouse
Host: Mouse
Application: IHC, ICC, WB

Product Name

Anti-Fibronectin Fn1 Monoclonal Antibody

See all Fibronectin products

SKU/Catalog Number

MA1116

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fibronectin Fn1 Monoclonal Antibody catalog # MA1116. Tested in IHC, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fibronectin Fn1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1116)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

FN-15

Isotype

Mouse IgG1

Immunogen

Fibronectin from human plasma.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1116 is reactive to Fn1 in Chicken, Human, Mouse

Applications

MA1116 is guaranteed for IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, chicken, mouse, By Heat
Immunocytochemistry , 1μg/ml, Human, chicken, mouse, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, mouse, -
Western blot, 1-2μg/ml, Human, chicken, mouse

Validation Images & Assay Conditions

Gene/Protein Information For Fn1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04937

Gene ID

25661

Gene Name

Fn1

Full Name

Fibronectin

Weight

272.511kDa

Alternative Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF Fn1|FIBNEC, fn-1|fibronectin 1|fibronectin|FN|fibronectin 2|fibronectin 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fn1, check out the Fn1 Infographic

Fn1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fn1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1116 has been cited in 25 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang X,Ke PX,Yuan X,Zhang GP,Chen WL,Zhang GS. Forskolin Protected against Streptozotocin-Induced Diabetic Cardiomyopathy via Inhibition of Oxidative Stress and Cardiac Fibrosis in Mice. Biomed Res Int.2021 Jan 29;2021:8881843.doi:10.1155/2021/8881843.PM
Species: Mouse
MA1116 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:NA

Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
Species: Human
MA1116 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:2000

Sang R,Liu Y,Kong L,Qian L,Liu C.Effect of Acellular Amnion With Increased TGF-β and bFGF Levels on the Biological Behavior of Tenocytes.Front Bioeng Biotechnol.2020 May 14;8:446.doi:10.3389/fbioe.2020.00446. PMID:32478059;PMCID:PMC7240037.
Species: Leghorn chicken
MA1116 usage in article: APP:IF, SAMPLE:ACELLULAR AMNIOTIC MEMBRANE, DILUTION:1:100

Glyceraldehyde-3-phosphate dehydrogenase acts as an adhesin in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells and as a receptor in %u2026

Rosiglitazone prevents advanced glycation end products%u2013induced renal toxicity likely through suppression of plasminogen activator inhibitor-1

Contribution of fibronectin-binding protein to pathogenesis of Streptococcus equi ssp . zooepidemicus

Elongation Factor Thermo Unstable (EF-Tu) Moonlights as an Adhesin on the Surface of Mycoplasma hyopneumoniae by Binding to Fibronectin

Pathophysiology of chronic pancreatitis induced by dibutyltin dichloride joint ethanol in mice

Wu Y, Wang Y, Ji Y, Ou Y, Xia H, Zhang B, Zhao Y. Am J Transl Res. 2017 Dec 15;9(12):5708-5718. eCollection 2017. C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells

Fu Jr, Liu Wl, Zhou Yf, Zhou Jf, Sun Hy, Luo L, Zhang H, Xu Hz. Chin Med J (Engl). 2005 Dec 5;118(23):1979-86. Expansive Effects Of Aorta-Gonad-Mesonephros-Derived Stromal Cells On Hematopoietic Stem Cells From Embryonic Stem Cells.

Xue H, Zhang Yl, Liu Gs, Wang H. J Pharmacol Exp Ther. 2005 Nov;315(2):501-9. Epub 2005 Jul 28. A New Atp-Sensitive Potassium Channel Opener Protects The Kidney From Hypertensive Damage In Spontaneously Hypertensive Rats.

Yang Y, Gao X, Wang X, Su L, Xing H. Eur J Pharm Sci. 2012 Aug 30;47(1):75-83. Doi: 10.1016/J.Ejps.2012.05.005. Epub 2012 May 23. Total Flavonoids Of Fructus Chorspondiatis Inhibits Collagen Synthesis Of Cultured Rat Cardiac Fibroblasts Induced By...

Guo Y, Zeng Qc, Zhang Cq, Zhang Xz, Li Rx, Wu Jm, Guan J, Liu L, Zhang Xc, Li Jy, Wan Zm. Int J Med Sci. 2013 Nov 5;10(13):1837-45. Doi: 10.7150/Ijms.6786. Ecollection 2013. Extracellular Matrix Of Mechanically Stretched Cardiac Fibroblasts Improv...

Wang H, Wang Hs, Zhou Bh, Li Cl, Zhang F, Wang Xf, Zhang G, Bu Xz, Cai Sh, Du J. Plos One. 2013;8(2):E56664. Doi: 10.1371/Journal.Pone.0056664. Epub 2013 Feb 19. Epithelial-Mesenchymal Transition (Emt) Induced By Tnf-?? Requires Akt/Gsk-3??-Mediat...

Shi Z, Shen T, Liu Y, Huang Y, Jiao J. J Biol Chem. 2014 Mar 7;289(10):6415-28. Doi: 10.1074/Jbc.M113.515601. Epub 2014 Jan 23. Retinoic Acid Receptor ?? (Rarg) And Nuclear Receptor Subfamily 5, Group A, Member 2 (Nr5A2) Promote Conversion Of Fibr...

Gai X, Lu Z, Tu K, Liang Z, Zheng X. Plos One. 2014 Jan 13;9(1):E84551. Doi: 10.1371/Journal.Pone.0084551. Ecollection 2014. Caveolin-1 Is Up-Regulated By Gli1 And Contributes To Gli1-Driven Emt In Hepatocellular Carcinoma.

Xia H, Cheung Wk, Sze J, Lu G, Jiang S, Yao H, Bian Xw, Poon Ws, Kung Hf, Lin Mc. J Biol Chem. 2010 Nov 19;285(47):36995-7004. Doi: 10.1074/Jbc.M110.133744. Epub 2010 Sep 7. Mir-200A Regulates Epithelial-Mesenchymal To Stem-Like Transition Via Zeb...

Li T, Yang Z, Cai Hj, Song Lw, Lu Ky, Zhou Z, Wu Zd. World J Gastroenterol. 2010 Feb 14;16(6):749-54. Effects Of In Vitro Cultivated Calculus Bovis Compound On Pulmonary Lesions In Rabbits With Schistosomiasis.

Cheng Y, Wang Y, Kang Yz, Hu Py, Gao Y, Pan Mx. Digestion. 2013;87(3):189-95. Doi: 10.1159/000349949. Epub 2013 May 1. In Vitro Culture Of Tumour-Derived Hepatocytes In Decellularised Whole-Liver Biological Scaffolds.

Wu Y, Chen W, Guo M, He Q, Hu Y. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4827-36. Ecollection 2014. Effects Of Transforming Growth Factor-??2 On Myocilin Expression And Secretion In Human Primary Cultured Trabecular Meshwork Cells.

Gong X, Liu H, Ding X, Liu M, Li X, Zheng L, Jia X, Zhou G, Zou Y, Li J, Huang X, Fan Y. Biomaterials. 2014 Jun;35(17):4782-91. Doi: 10.1016/J.Biomaterials.2014.02.050. Epub 2014 Mar 15. Physiological Pulsatile Flow Culture Conditions To Generate ...

Xiao H, Ji Am, Li Zl, Song Xd, Su D, Chen Ah. Sheng Li Xue Bao. 2008 Jun 25;60(3):355-61. Interleukin-1?? Inhibits Collagen Synthesis And Promotes Its Decomposition In Cultured Cardiac Fibroblasts.

Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.

Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...

Sasaki M, Inoue M, Katada Y, Taguchi T. J Mater Sci Mater Med. 2013 Apr;24(4):951-8. Doi: 10.1007/S10856-012-4845-6. Epub 2013 Jan 19. Promotion Of Initial Cell Adhesion On Trisuccinimidyl Citrate-Modified Nickel-Free High-Nitrogen Stainless Steel.

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16 Customer Q&As for Anti-Fibronectin Fn1 Monoclonal Antibody

Question

We want to test anti-Fibronectin Monoclonal antibody MA1116 on chicken urine for research purposes, then I may be interested in using anti-Fibronectin Monoclonal antibody MA1116 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Evans

Verified customer

Asked: 2020-04-09

Answer

The products we sell, including anti-Fibronectin Monoclonal antibody MA1116, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-09

Question

I was wanting to use your anti-Fibronectin Monoclonal antibody for IHC for chicken urine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for chicken urine identification?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

You can see on the product datasheet, MA1116 anti-Fibronectin Monoclonal antibody has been validated for IHC, ICC, WB on chicken, human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in chicken urine in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-13

Question

Does MA1116 anti-Fibronectin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

It shows on the product datasheet, MA1116 anti-Fibronectin Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-08

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urine using anti-Fibronectin Monoclonal antibody MA1116. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-27

Question

Is this MA1116 anti-Fibronectin Monoclonal antibody reactive to the isotypes of FN1?

Verified Customer

Verified customer

Asked: 2019-09-09

Answer

The immunogen of MA1116 anti-Fibronectin Monoclonal antibody is Fibronectin from human plasma. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-09

Question

See attached the WB image, lot number and protocol we used for urine using anti-Fibronectin Monoclonal antibody MA1116. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-14

Question

Is a blocking peptide available for product anti-Fibronectin Monoclonal antibody (MA1116)?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

We do provide the blocking peptide for product anti-Fibronectin Monoclonal antibody (MA1116). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-18

Question

Is there a BSA free version of anti-Fibronectin Monoclonal antibody MA1116 available?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Fibronectin Monoclonal antibody MA1116 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-05

Question

Does anti-Fibronectin Monoclonal antibody MA1116 work on monkey WB with retinal pigment epithelium?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

Our lab technicians have not validated anti-Fibronectin Monoclonal antibody MA1116 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Fibronectin Monoclonal antibody MA1116 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey retinal pigment epithelium in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-11

Question

We have been able to see staining in chicken fibroblast. What should we do? Is anti-Fibronectin Monoclonal antibody supposed to stain fibroblast positively?

Verified Customer

Verified customer

Asked: 2019-04-18

Answer

According to literature fibroblast does express FN1. According to Uniprot.org, FN1 is expressed in tendon, retinal pigment epithelium, aortic endothelium, amygdala, cervix, colon endothelium, endometrial tumor rc uterine endothelium, mammary cancer, fibroblast, plasma, peripheral blood t-cell umbilical vein endothelial cell, urine, cartilage, mammary carcinoma, cervix carcinoma, liver, among other tissues. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963

Boster Scientific Support

Answered: 2019-04-18

Question

My team were happy with the WB result of your anti-Fibronectin Monoclonal antibody. However we have seen positive staining in retinal pigment epithelium secreted using this antibody. Is that expected? Could you tell me where is FN1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-07-18

Answer

From what I have seen in literature, retinal pigment epithelium does express FN1. Generally FN1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963

Boster Scientific Support

Answered: 2018-07-18

Question

I have a question about product MA1116, anti-Fibronectin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1116 anti-Fibronectin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-07-11

Question

We purchased anti-Fibronectin Monoclonal antibody for WB on urine in the past. I am using human, and We are going to use the antibody for IHC next. We want examining urine as well as tendon in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

E. Patel

Verified customer

Asked: 2018-04-06

Answer

I have checked the website and datasheets of our anti-Fibronectin Monoclonal antibody and it appears that MA1116 has been validated on human in both WB and IHC. Thus MA1116 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-04-06

Question

I see that the anti-Fibronectin Monoclonal antibody MA1116 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-12-05

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-12-05

Question

Would anti-Fibronectin Monoclonal antibody MA1116 work for IHC with urine?

W. Gonzalez

Verified customer

Asked: 2014-11-24

Answer

According to the expression profile of urine, FN1 is highly expressed in urine. So, it is likely that anti-Fibronectin Monoclonal antibody MA1116 will work for IHC with urine.

Boster Scientific Support

Answered: 2014-11-24

Question

We are currently using anti-Fibronectin Monoclonal antibody MA1116 for chicken tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says chicken, human, mouse. Is it possible that the antibody can work on pig tissues as well?

C. Moore

Verified customer

Asked: 2014-09-11

Answer

The anti-Fibronectin Monoclonal antibody (MA1116) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-09-11

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MA1116
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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