|Validated Species:||Chicken, Human, Mouse|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-Fibronectin Monoclonal Antibody|
|Description||Mouse IgG monoclonal antibody for Fibronectin, fibronectin 1 (FN1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Fibronectin Monoclonal Antibody FN-15 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1116)|
|Replacement Item||This antibody may replace the following items: sc-18825|sc-18826|sc-18827|sc-271098|sc-50078|sc-50079|sc-50080|sc-50081|sc-52331|sc-53285|sc-53286|sc-59824|sc-59826|sc-6952|sc-6953|sc-69681|sc-69682|sc-73611|sc-81767|sc-81769|sc-8422|sc-9068|sc-98454 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Mouse|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Fibronectin from human plasma.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||272511 MW|
|Protein Function||Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). .|
|Tissue Specificity||Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.|
|Sequence Similarities||Contains 12 fibronectin type-I domains.|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix.|
Background for Fibronectin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Fibronectin Monoclonal Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, chicken, mouse, By Heat
Immunocytochemistry , 1μg/ml, Human, chicken, mouse, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, chicken, mouse, -
Western blot, 1-2μg/ml, Human, chicken, mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.