radio
SKU MA1116
Size 100μg/vial
Reactivity Chicken, Human, Mouse
Clonality Monoclonal
Clone Number FN-15
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, ICC, WB

Overview

Product Name Anti-Fibronectin Monoclonal Antibody
SKU/Catalog Number MA1116
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Fibronectin, fibronectin 1 (FN1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Fibronectin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1116)
Host Mouse
Immunogen Fibronectin from human plasma.
Reactivity Chicken, Human, Mouse

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, chicken, mouse, By Heat
Immunocytochemistry , 1μg/ml, Human, chicken, mouse, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, chicken, mouse, -
Western blot, 1-2μg/ml, Human, chicken, mouse

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P04937
Gene Name Fn1
Protein Name Fibronectin
Tissue Specificity Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
Alternative Names Fibronectin;FN;Anastellin;Fn1;
Subcellular Localization Secreted, extracellular space, extracellular matrix.
Molecular Weight 272511 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). .
Background Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.

Order Product (MA1116)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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We promise all of our products perform as described in datasheets.

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Publications

Wu Y, Wang Y, Ji Y, Ou Y, Xia H, Zhang B, Zhao Y. Am J Transl Res. 2017 Dec 15;9(12):5708-5718. eCollection 2017. C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells
Fu Jr, Liu Wl, Zhou Yf, Zhou Jf, Sun Hy, Luo L, Zhang H, Xu Hz. Chin Med J (Engl). 2005 Dec 5;118(23):1979-86. Expansive Effects Of Aorta-Gonad-Mesonephros-Derived Stromal Cells On Hematopoietic Stem Cells From Embryonic Stem Cells.
Xue H, Zhang Yl, Liu Gs, Wang H. J Pharmacol Exp Ther. 2005 Nov;315(2):501-9. Epub 2005 Jul 28. A New Atp-Sensitive Potassium Channel Opener Protects The Kidney From Hypertensive Damage In Spontaneously Hypertensive Rats.
Yang Y, Gao X, Wang X, Su L, Xing H. Eur J Pharm Sci. 2012 Aug 30;47(1):75-83. Doi: 10.1016/J.Ejps.2012.05.005. Epub 2012 May 23. Total Flavonoids Of Fructus Chorspondiatis Inhibits Collagen Synthesis Of Cultured Rat Cardiac Fibroblasts Induced By...
Guo Y, Zeng Qc, Zhang Cq, Zhang Xz, Li Rx, Wu Jm, Guan J, Liu L, Zhang Xc, Li Jy, Wan Zm. Int J Med Sci. 2013 Nov 5;10(13):1837-45. Doi: 10.7150/Ijms.6786. Ecollection 2013. Extracellular Matrix Of Mechanically Stretched Cardiac Fibroblasts Improv...
Wang H, Wang Hs, Zhou Bh, Li Cl, Zhang F, Wang Xf, Zhang G, Bu Xz, Cai Sh, Du J. Plos One. 2013;8(2):E56664. Doi: 10.1371/Journal.Pone.0056664. Epub 2013 Feb 19. Epithelial-Mesenchymal Transition (Emt) Induced By Tnf-?? Requires Akt/Gsk-3??-Mediated...
Shi Z, Shen T, Liu Y, Huang Y, Jiao J. J Biol Chem. 2014 Mar 7;289(10):6415-28. Doi: 10.1074/Jbc.M113.515601. Epub 2014 Jan 23. Retinoic Acid Receptor ?? (Rarg) And Nuclear Receptor Subfamily 5, Group A, Member 2 (Nr5A2) Promote Conversion Of Fibro...
Gai X, Lu Z, Tu K, Liang Z, Zheng X. Plos One. 2014 Jan 13;9(1):E84551. Doi: 10.1371/Journal.Pone.0084551. Ecollection 2014. Caveolin-1 Is Up-Regulated By Gli1 And Contributes To Gli1-Driven Emt In Hepatocellular Carcinoma.
Xia H, Cheung Wk, Sze J, Lu G, Jiang S, Yao H, Bian Xw, Poon Ws, Kung Hf, Lin Mc. J Biol Chem. 2010 Nov 19;285(47):36995-7004. Doi: 10.1074/Jbc.M110.133744. Epub 2010 Sep 7. Mir-200A Regulates Epithelial-Mesenchymal To Stem-Like Transition Via Zeb...
Cheng Y, Wang Y, Kang Yz, Hu Py, Gao Y, Pan Mx. Digestion. 2013;87(3):189-95. Doi: 10.1159/000349949. Epub 2013 May 1. In Vitro Culture Of Tumour-Derived Hepatocytes In Decellularised Whole-Liver Biological Scaffolds.
Wu Y, Chen W, Guo M, He Q, Hu Y. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4827-36. Ecollection 2014. Effects Of Transforming Growth Factor-??2 On Myocilin Expression And Secretion In Human Primary Cultured Trabecular Meshwork Cells.
Gong X, Liu H, Ding X, Liu M, Li X, Zheng L, Jia X, Zhou G, Zou Y, Li J, Huang X, Fan Y. Biomaterials. 2014 Jun;35(17):4782-91. Doi: 10.1016/J.Biomaterials.2014.02.050. Epub 2014 Mar 15. Physiological Pulsatile Flow Culture Conditions To Generate ...
Xiao H, Ji Am, Li Zl, Song Xd, Su D, Chen Ah. Sheng Li Xue Bao. 2008 Jun 25;60(3):355-61. Interleukin-1?? Inhibits Collagen Synthesis And Promotes Its Decomposition In Cultured Cardiac Fibroblasts.
Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.
Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...
Sasaki M, Inoue M, Katada Y, Taguchi T. J Mater Sci Mater Med. 2013 Apr;24(4):951-8. Doi: 10.1007/S10856-012-4845-6. Epub 2013 Jan 19. Promotion Of Initial Cell Adhesion On Trisuccinimidyl Citrate-Modified Nickel-Free High-Nitrogen Stainless Steel.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to fibronectin 1 antibody, fn1 antibody, msf antibody