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Product Info Summary
| SKU: | A02492-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-HSPB8/HSP22 Monoclonal Antibody
SKU/Catalog Number
A02492-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HSPB8/HSP22 Monoclonal Antibody catalog # A02492-1. Tested in WB applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSPB8/HSP22 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02492-1)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
2C3
Isotype
IgG1
Immunogen
Recombinant Protein of HSPB8/HSP22
Reactive Species
A02492-1 is reactive to HSPB8 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
21.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02492-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-2000
Validation Images & Assay Conditions
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Western blot analysis of 293T with HSPB8/HSP22 Mouse mAb diluted at 1:2, 000.
Specific Publications For Anti-HSPB8/HSP22 Monoclonal Antibody (A02492-1)
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Customer Q&As
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7 Customer Q&As for Anti-HSPB8/HSP22 Monoclonal Antibody
Question
Is there a BSA free version of anti-HSPB8/HSP22 Monoclonal antibody A02492-1 available?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HSPB8/HSP22 Monoclonal antibody A02492-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-25
Question
I have attached the WB image, lot number and protocol we used for thalamus using anti-HSPB8/HSP22 Monoclonal antibody A02492-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-16
Question
Would A02492-1 anti-HSPB8/HSP22 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
It shows on the product datasheet, A02492-1 anti-HSPB8/HSP22 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-15
Question
Is a blocking peptide available for product anti-HSPB8/HSP22 Monoclonal antibody (A02492-1)?
E. Wu
Verified customer
Asked: 2014-05-14
Answer
We do provide the blocking peptide for product anti-HSPB8/HSP22 Monoclonal antibody (A02492-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-05-14
Question
Is this A02492-1 anti-HSPB8/HSP22 Monoclonal antibody reactive to the isotypes of HSPB8?
T. Li
Verified customer
Asked: 2014-02-07
Answer
The immunogen of A02492-1 anti-HSPB8/HSP22 Monoclonal antibody is Recombinant Protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-07
Question
I see that the anti-HSPB8/HSP22 Monoclonal antibody A02492-1 works with WB, what is the protocol used to produce the result images on the product page?
S. Yang
Verified customer
Asked: 2013-10-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-10-07
Question
Would anti-HSPB8/HSP22 Monoclonal antibody A02492-1 work for WB with thalamus?
A. Patel
Verified customer
Asked: 2013-08-01
Answer
According to the expression profile of thalamus, HSPB8 is highly expressed in thalamus. So, it is likely that anti-HSPB8/HSP22 Monoclonal antibody A02492-1 will work for WB with thalamus.
Boster Scientific Support
Answered: 2013-08-01
