Anti-Hu CD147 Purified BSG Monoclonal Antibody

Product Name

Anti-Hu CD147 Purified BSG Monoclonal Antibody

View all BSG Antibodies

SKU/Catalog Number

M00248

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD147 Purified BSG Monoclonal Antibody (Catalog# M00248). Tested in Flow Cytometry, IP, WB, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD147 Purified BSG Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00248)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-M6/1

Isotype

Mouse IgG1

Immunogen

Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer). The antibody MEM-M6/1 recognizes an extracellular epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

Reactive Species

M00248 is reactive to BSG in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

42.2 kDa

Background of BSG

CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00248 is guaranteed for Flow Cytometry, IP, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: testis.
Flow cytometry: 10 μg/ml.
Western blotting: 1 μg/ml, both reducing and non-reducing conditions

Validation Images & Assay Conditions

See full target information BSG

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