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Product Info Summary
| SKU: | M00248-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD147 BSG Rabbit Monoclonal Antibody
SKU/Catalog Number
M00248-1
BM4979 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD147 BSG Rabbit Monoclonal Antibody catalog # M00248-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD147 BSG Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00248-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAOC-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD147
Reactive Species
M00248-1 is reactive to BSG in Human, Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
42.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00248-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human SiHa whole cell, human Hela whole cell, human HepG2 whole cell, rat kidney tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue
IHC: human stomach cancer tissue
ICC/IF: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of CD147 using anti-CD147 antibody (M00248-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147 antigen affinity purified monoclonal antibody (Catalog # M00248-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD147 at approximately 65 kDa. The expected band size for CD147 is at 42 kDa.
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Immunohistochemical analysis of paraffin-embedded human stomach cancer, using CD147 Antibody.
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Immunofluorescent analysis of Jurkat cells, using using CD147 Antibody.
Specific Publications For Anti-CD147 BSG Rabbit Monoclonal Antibody (M00248-1)
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