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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IF, IHC, ICC, WB|
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Anti-CD147 BSG Rabbit Monoclonal Antibody
Boster Bio Anti-CD147 BSG Rabbit Monoclonal Antibody catalog # M00248-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD147 BSG Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00248-1)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human CD147
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M00248-1 is reactive to BSG in Human, Mouse, Rat
M00248-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Western blot analysis of CD147 expression in Mouse kidney lysate (M00248-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BSG monoclonal antibody (Catalog # M00248-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BSG
Gene/Protein Information For BSG (Source: Uniprot.Org, NCBI)
5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor BSG 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, SLC7A11, TCSF basigin (Ok blood group) basigin|OK blood group antigen|collagenase stimulatory factor|extracellular matrix metalloproteinase inducer|hepatoma-associated antigen|leukocyte activation antigen M6|tumor cell-derived collagenase stimulatory factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on BSG, check out the BSG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BSG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M00248-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lu Q, Lv G, Kim A, Ha Jm, Kim S. Oncol Lett. 2013 Jan;5(1):201-207. Epub 2012 Oct 19. Expression And Clinical Significance Of Extracellular Matrix Metalloproteinase Inducer, Emmprin/Cd147, In Human Osteosarcoma.
Ying Hz, Liu Yh, Yu B, Wang Zy, Zang Jn, Yu Ch. Food Chem Toxicol. 2013 Feb;52:53-60. Doi: 10.1016/J.Fct.2012.10.030. Epub 2012 Nov 2. Dietary Quercetin Ameliorates Nonalcoholic Steatohepatitis Induced By A High-Fat Diet In Gerbils.
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