Click image to see more details
Product Info Summary
| SKU: | M06261-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-F, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hu CD266 Purified TNFRSF12A Monoclonal Antibody
SKU/Catalog Number
M06261-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD266 Purified TNFRSF12A Monoclonal Antibody (Catalog# M06261-1). Tested in Flow Cytometry, WB, IHC-F application(s). This antibody reacts with Mouse, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD266 Purified TNFRSF12A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06261-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
ITEM-4
Isotype
Mouse IgG2b
Immunogen
Human CD266-transfected P815 cells. The mouse monoclonal antibody ITEM-4 recognizes an extracellular epitope of CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis.
Cross-reactivity
Cross-reactivity with CLIC5b was not determined.
Reactive Species
M06261-1 is reactive to TNFRSF12A in Human, Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
13.9 kDa
Background of TNFRSF12A
CD266 / TWEAK R (TNFRSF12A), also known as FN14 (fibroblast growth factor-inducible 14) is a receptor for CD255 / TWEAK, the TNF-like weak inducer of apoptosis. CD266 is expressed on endothelial cells, as well as on some cancer tissues, and plays a role in CD255-induced endothelial cell migration, proliferation, and angiogenesis. The CD255-CD266 interaction, or antibody-mediated triggering of CD266 is also able to induce apoptosis and necrosis in CD266-positive cells (including tumor cells), which might have therapeutic potential.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06261-1 is guaranteed for Flow Cytometry, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Separation of HUVEC cells stained using anti-human CD266 (ITEM-4) purified antibody (concentration in sample 1 µg/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Click image to see more details
Flow cytometry surface staining pattern of HUVEC cells stained using anti-human CD266 (ITEM-4) purified antibody (concentration in sample 1 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD266 Purified TNFRSF12A Monoclonal Antibody (M06261-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hu CD266 Purified TNFRSF12A Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Hu CD266 Purified TNFRSF12A Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
