Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine™

Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf12a in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1754
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Product Name

Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine™

See all TWEAK R/TNFRSF12 products

SKU/Catalog Number

EK1754

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf12a in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1754)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E28-P80

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1754 is reactive to Tnfrsf12a in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Application Guarantee

EK1754 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1754-CAP Anti-Mouse Tnfrsf12a Pre-coated 96-well strip microplate 1
EK1754-ST Mouse Tnfrsf12a Standard 2 vials, 10 ng/tube
EK1754-DA Mouse Tnfrsf12a Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0240.1950.3260.5441.0771.6892.3362.436

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TWEAK R/TNFRSF12, Dilution ratio of 1:1, concentration in serum is around 2ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3002167478930723794841
Standard deviation18.3143.02258.613.2114.19363.08
CV (%)6.1%6.6%5.4%4.3%4.8%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TWEAK R/TNFRSF12 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 130032530833031512.213.8%
Sample 22167217620722103212943.522.0%
Sample 347894265416549334538328.897.2%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf12a (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9CR75

Gene ID

27279

Gene Name

Tnfrsf12a

Full Name

Tumor necrosis factor receptor superfamily member 12A

Weight

13.641kDa

Alternative Names

CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14 Tnfrsf12a|AI255180, C87282, Fn1, Fn14, HPIP, TWEAK, TWEAK-R, Twea, TweakR|tumor necrosis factor receptor superfamily, member 12a|tumor necrosis factor receptor superfamily member 12A|FGF-inducible 14|fibroblast growth factor-inducible immediate-early response protein 14|fibroblast growth factor-regulated protein 2|tweak-receptor|type I transmembrane protein Fn14

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf12a, check out the Tnfrsf12a Infographic

Tnfrsf12a infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf12a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine™

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

K. Roberts

Verified customer

Asked: 2020-03-28

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-03-28

Question

Q: if the enzyme conjugated TWEAK R antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TWEAK R antigen?

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-03-16

Question

Q: is it okay to use EDTA plasma as samples in Mouse TWEAK R Picokine® ELISA Kit (Catalog # EK1754)?

H. Williams

Verified customer

Asked: 2019-11-15

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TWEAK R causing degradation of its protein structure. TWEAK R may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TWEAK R ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-11-15

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

A. Mitchell

Verified customer

Asked: 2019-03-03

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-03-03

Question

Q: how to proceed with the analysis of ELISA data? I have obtained TWEAK R level in serum.

T. Campbell

Verified customer

Asked: 2019-01-26

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-01-26

Question

Q: What is the optimal O.D. value for TWEAK R ELISA kit? I used your TWEAK R ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TWEAK R even though the O.D. values are not very high?

R. Kulkarni

Verified customer

Asked: 2018-05-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TWEAK R ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of TWEAK R that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2018-05-27

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-01-23

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-01-23

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