Click image to see more details
Product Info Summary
| SKU: | M11226 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hu CD300e Purified Monoclonal Antibody
SKU/Catalog Number
M11226
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD300e Purified Monoclonal Antibody (Catalog# M11226). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD300e Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11226)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
UP-H2
Isotype
Mouse IgG1 kappa
Immunogen
CD300e-HA-transfected cells. The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M11226 is reactive to CD300E in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
22.9 kDa
Background of CD300E
CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11226 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
Click image to see more details
Separation of human monocytes (red-filled) from CD300e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD300e (UP-H2) purified antibody (concentration in sample 4 µg/ml, GAM APC).
Click image to see more details
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD300e (UP-H2) purified antibody (concentration in sample 4 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD300e Purified Monoclonal Antibody (M11226)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hu CD300e Purified Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Hu CD300e Purified Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
