Human CD300E ELISA Kit PicoKine®

CD300e/LMIR6 ELISA kit for Human

Human CD300E ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD300E in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2154
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human CD300E ELISA Kit PicoKine®

View all CD300e/LMIR6 ELISA kits

SKU/Catalog Number

EK2154

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD300E ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD300E in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CD300E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2154)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: HEK293; Immunogen sequence: L18-L169

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2154 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2154 is reactive to Cd300e in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2154 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CD300e/LMIR6

This gene encodes a member of the CD300 glycoprotein family of cell surface proteins expressed on myeloid cells. The protein interacts with the TYRO protein tyrosine kinase-binding protein and is thought to act as an activating receptor.

Kit Components

Catalog Number Description Quantity
EK2154-CAP Anti-Human Cd300e Pre-coated 96-well strip microplate 1
EK2154-ST Human Cd300e Standard 2 vials, 10 ng/tube
EK2154-DA Human Cd300e Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)015.631.262.51252505001,000
O.D.0.0140.0560.1080.2770.4610.8701.6522.319

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD300E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)84336684106396723
Standard deviation4.720.1634.885.1913.0737.6
CV(%)5.6%6%5.1%4.9%3.3%5.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD300e/LMIR6 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 184858892872.883.3%
Sample 23363423653513497.322.1%
Sample 368472169668269624.353.5%
*number of samples for each test n=16.

Gene/Protein Information For Cd300e (Source: Uniprot.Org, NCBI)

Gene Name

Cd300e

Full Name

CMRF35-like molecule 2

Weight

21.822kDa

Superfamily

CD300 family

Alternative Names

CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cd300e, check out the Cd300e Infographic

Cd300e infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cd300e: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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