Anti-CD300e/Lmir6 Antibody

CD300e/LMIR6 antibody

Boster Bio Anti-CD300e/Lmir6 Antibody catalog # A11226. Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A11226
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: IHC

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Product Name

Anti-CD300e/Lmir6 Antibody

View all CD300e/LMIR6 Antibodies

SKU/Catalog Number

A11226

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CD300e/Lmir6 Antibody catalog # A11226. Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD300e/Lmir6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11226)

Host

Rabbit

Contents

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Polyclonal

Clone Number

3A8

Isotype

IgG

Immunogen

Synthetic peptide from human protein at AA range: 31-80.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11226 is reactive to Cd300e in Human

Applications

A11226 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21.822kDa

Background of CD300e/LMIR6

Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).

Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
Valtorta, F. et al. (2004) Bioessays 26, 445-53.
Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
Bonanomi, D. et al. (2007) Biochem J 404, 525-34.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC-P, 1:50-300

Validation Images & Assay Conditions

Gene/Protein Information For Cd300e (Source: Uniprot.org, NCBI)

Gene Name

Cd300e

Full Name

CMRF35-like molecule 2

Weight

21.822kDa

Superfamily

CD300 family

Alternative Names

CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cd300e, check out the Cd300e Infographic

Cd300e infographic

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In this infographic, you will see the following information for Cd300e: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-CD300e/Lmir6 Antibody

Question

Is a blocking peptide available for product anti-CD300e/Lmir6 antibody (A11226)?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

We do provide the blocking peptide for product anti-CD300e/Lmir6 antibody (A11226). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-04

Question

My question regards to test anti-CD300e/Lmir6 antibody A11226 on human peripheral blood monocyte for research purposes, then I may be interested in using anti-CD300e/Lmir6 antibody A11226 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

The products we sell, including anti-CD300e/Lmir6 antibody A11226, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-20

Question

I was wanting to use your anti-CD300e/Lmir6 antibody for IHC for human peripheral blood monocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human peripheral blood monocyte identification?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

You can see on the product datasheet, A11226 anti-CD300e/Lmir6 antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human peripheral blood monocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-09

Question

We are currently using anti-CD300e/Lmir6 antibody A11226 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-23

Answer

The anti-CD300e/Lmir6 antibody (A11226) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-23

Question

Does A11226 anti-CD300e/Lmir6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Johnson

Verified customer

Asked: 2018-02-13

Answer

You can see on the product datasheet, A11226 anti-CD300e/Lmir6 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-02-13

Question

I see that the anti-CD300e/Lmir6 antibody A11226 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-01-30

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-01-30

Question

Is this A11226 anti-CD300e/Lmir6 antibody reactive to the isotypes of CD300E?

N. Banerjee

Verified customer

Asked: 2013-08-15

Answer

The immunogen of A11226 anti-CD300e/Lmir6 antibody is Synthetic peptide from human protein at AA range: 31-80. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-08-15

Order DetailsPrice
A11226

100uL

$399

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