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Product Info Summary
| SKU: | M02073-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-F |
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Product info
Product Name
Anti-Hu CD42b Purified GP1BA Monoclonal Antibody
SKU/Catalog Number
M02073-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (Catalog# M02073-1). Tested in Flow Cytometry, IHC-F application(s). This antibody reacts with Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02073-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
HIP1
Isotype
Mouse IgG1
Immunogen
Peripheral blood mononuclear cells of a patient suffering with CLL. The antibody HIP1 reacts with an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02073-1 is reactive to GP1BA in Human, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
71.5 kDa
Background of GP1BA
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02073-1 is guaranteed for Flow Cytometry, IHC-F Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Separation of human thrombocytes (red-filled) from CD42b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD42b (HIP1) purified antibody (concentration in sample 4 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD42b (HIP1) purified antibody (concentration in sample 4 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD42b Purified GP1BA Monoclonal Antibody (M02073-1)
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