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Product Info Summary
| SKU: | M00171-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Cow, Human, Rat |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IHC-P, ICC, WB |
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Product info
Product Name
Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody
SKU/Catalog Number
M00171-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody (Catalog# M00171-2). Tested in WB, IHC-P, ELISA, Flow Cytometry, ICC application(s). This antibody reacts with Human, Cow, Rat.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00171-2)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
MEM-111
Isotype
Mouse IgG2a
Immunogen
Raji human Burkitt's lymphoma cell line. The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
Reactive Species
M00171-2 is reactive to ICAM1 in Cow, Human, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
57.8 kDa
Background of ICAM1
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00171-2 is guaranteed for ELISA, Flow Cytometry, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 0.5-4 μg/ml.
Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: thymus, RE cells.
Western blotting: Positive control: RAJI cell line K562 leukemia cell line, JY cell line, aa ctivated T-lymphocytes, non-reducing conditions.
Validation Images & Assay Conditions
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Western blotting analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111. Lower bands represent tubulin as a loading control.
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Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 (MEM-15) PE antibody (20 µl reagent / 100 µl of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).
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Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).
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Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD54 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD54 at approximately 88 kDa.
Specific Publications For Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody (M00171-2)
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