ICAM1 Antibodies

Intercellular adhesion molecule 1 (ICAM1)

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). Throughout leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.

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Gene Information

Human
Gene Name:	ICAM1
Uniprot:	P05362
Entrez:	3383

Family

Belongs to:
immunoglobulin superfamily

Alternative Names

BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Molecular Weight

Mass (kDA):
57.825 kDA

Genomic Context

Human
Location:	19p13.2
Sequence:	19; NC_000019.10 (10271120..10286615)

Subcellular Localizations

Membrane; Single-pass type I membrane protein.

Diseases

• Tissue Adhesions
• Inflammation
• Malignant Neoplasms
• Neoplasms
• Atherosclerosis
• Inflammatory Response
• Ischemia
• Diabetes Mellitus
• Cardiovascular Diseases
• Autoimmune Reaction
• Tumor Angiogenesis
• Infarction

• Malaria
• Carcinoma
• Neoplasm Metastasis
• Reperfusion Injury
• Cell Invasion
• Myocardial Infarction
• Hypertensive Disease

Interacting Proteins

• ITGAL

Pathways

• Pathogenesis
• Cell Adhesion
• Immune Response
• Inflammatory Response
• Angiogenesis
• Secretion
• Cell Proliferation
• Cell Activation
• Reverse Transcription
• Cell Migration
• Localization
• Cell Death
• Endocytosis

• Aging
• Cell Cycle
• Antigenic Variation
• Chemotaxis
• Wound Healing
• Regeneration
• Transport

PTMs

• Phosphorylation
• Cleavage
• Methylation
• Reduction

• Acetylation
• Ubiquitination
• Demethylation
• Biotinylation

• Deacetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ICAM1

Best Uses Of The ICAM1 Marker

ICAM1 is a transmembrane glycoprotein that plays an important role in cell biology. Its transmembrane structure allows it to easily detect cells and measure their numbers in a fluid mix that is heterogeneous. Boster Bio protocols for flow cytometry provide an easy-to-follow guideline of the flow cytometry process. These protocols are simple to follow and can be used as a guide to help you get started with this vital marker for biology. The detailed instructions on how to prepare the sample are provided along with product datasheets.

ICAM1 is a transmembrane-specific protein.

ICAM-1 is a transmembrane glycopeptide involved in adhesion of tumor cells to endothelial cells. Inflammatory factors stimulate tumor cells to produce ICAM-1. It stimulates the release of growth factors and proinflammatory cytokines. Arteta et al. Arteta and colleagues. identified the roles of ICAM-1b and IL-1b as creating a favorable environment for the growth of tumors. ManR-mediated endocytosis also was associated with a lower cytotoxicity in lymphocytes in the liver of the murine cell line.

ICAM-1, a transmembrane glycopeptide, is composed of five extracellular immunoglobulin -like domains. It is found on a variety of kinds of cells, such as non-hematopoietic cells, endothelial cell, fibroblasts, and cancer cells. The biological effects of ICAM-1 have not been fully comprehended. Further research is needed to develop effective therapies.

ICAM1 is transmembranous glycoprotein, which functions as a ligand for b2-integrin molecules. Mice lacking ICAM1 show reduced lymphocyte recruitment to the lung. Airway inflammation and hyperresponsiveness also are decreased in these mice. In the hope of identifying the possible connection between ICAM1 variants and asthma, the researchers assessed ICAM1 levels in serum samples to determine whether ICAM1 is associated with asthma in paediatric patients.

Endothelial cells generate ICAM-1, a transmembrane protein which plays an important role in cancer cell adhesion. ICAM-1 expression in endothelial cells is associated with a distinct liver-leukocyte adhesion mechanism, which differs from other mechanisms for adhesion of leukocytes. In addition to encouraging tumor cell adhesion ICAM-1 expression is essential for the colonization of liver cancer cells.

The activation of ICAM-1 stimulates pro-angiogenic as well as pro-desmoplastic stromal cells, which promote the growth and growth of cancerous liver cells. HSCs are primary producers of ECM proteins in the liver and the presence of ICAM-1 in human CRC fibroblasts is associated with an increase in the number of desmoplasia sections. Further research can be conducted to understand how ICAM-1 impacts the inflammation response mediated by HSCs.

ICAM1 is expressed in human cancer cells. It is found in follicles comprised of CD20+ T cells as well as CD21+ FDCs. TLS-formed tumor cells display an aggressive phenotype. The luminal type showed higher levels of ICAM1-mRNA than those with TLS formation. These results suggest that ICAM1 could be involved in cancer metastasis.

Numerous studies have explored the importance of ICAM-1 as an mediator of inflammation. Mycobacterium tuberculosis, a bacteria that causes infection, alters the function and expression of adhesion molecules in monocytic cells. It also inhibits metastasis from tumors by preventing macrophage M2 polarization. Numerous studies have also demonstrated the connection between ICAM-1 polarization of macrophages.

ShRNA has been utilized to study the role of ICAM1 in the process of adhesion to extracellular cells. Inducible short hairpin-RNA, shRNA, is used to target the human ICAM1 gene. The target sequence of shRNA is GCTTCGCCGTAGTCTTA, and the lentiviral vector has four groups. To accomplish this, the vector's lentiviral expression is co-expressed with EGFP. ICAM1 was significantly decreased by shRNA-b. Cells that were cultured with shRNA-b exhibited smaller aggregates.

DS treatment reduced the expression of ICAM1 in the hPSCs. ICAM inhibition blocked adhesion between aggregates of hPSCs, and also reduced the size of their aggregates that were heterogeneous. This suggests that ICAM1 could play a key role in the determination of the size of aggregates. These results suggest that the transmembrane glycoprotein is involved in cell adhesion.

In addition to the transmembrane structure of the receptor, ICAM-1 also plays a significant role in macrophage phagocytosis. This is a result of the presence lipopolysaccharide molecules. ICAM-1-positive macrophages become less efficient in phagocytosis upon LPS stimulation. The effect of LPS on macrophage phagocytosis has been assessed using flow cytometry as well as Confocal microscopy.

Although the DS is a key factor in determining risk for CRC however it isn't sufficient. Both ICAM-1 K469E and ICAM-1 R241G genotypes are not associated with CRC susceptibility. However, heterozygous variants of ICAM-1 as well as IL-6-174 G-C showed no significant association with CRC risk.

It is a transmembrane glycoprotein

Described as an endothelial-leukocyte-associated transmembrane glycoprotein, ICAM1 is responsible for stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration. Although its role in the development of diabetes is still unclear, recent research shows that it is crucial in the pathogenesis and progression of disease. In this article, we'll outline the current research conducted on this gene and discuss the future possibilities for its use.

ICAM-1's structure and properties play a role in its role in intercellular adhesion. It also contains ligand-binding sites that enable it to bind with various molecules. The protein binds to fibrinogen, leukocyte function associated antigen-1 and macrophage adhesion mole-1 three molecules that are found on the surface of various cell types including endothelial cells.

ICAM-1 is also a key factor in the progression of tumors. It is found on the surface of liver cells that reside in the body and could be an ideal target for complementary treatments. It also acts as a transducer and molecule, creating a strong inflammation response that results increased extravasation and adhesion. It also promotes the recruitment and activation of immunoregulatory cells, which can reduce the immune surveillance of tumor cells.

ICAM1 is a transmembranous glycoprotein with roles in vascular epithelial cells and the sinusoidal endothelial cells, as well as in the central nervous system. It has multiple functions in the nervous system and is found in a variety of tissues, including the gray and white matter. It plays a key role in the biology and maintenance of psychiatric disorders.

In vitro research ICAM1 was found to be a crucial element in regulating adhesion of cells. It is essential for adhesion and extracellular signaling in 2D adhesion cells. Blocking ICAM1 reduced anti-CT4 expression and the expression of b2 Integrin. This suggests that ICAM1 could play an important role in controlling aggregate size in 3D suspension culture.

ICAM-1 is 90kD protein which is heavily glycosylated. It is composed of one transmembrane segment and five Ig-like extracellular domains and one transmembrane section. By removing the cytoplasmic tail of ICAM-1, its association with the actin cytoskeleton is eliminated. A truncated form of ICAM-1 was sufficient to allow its internalization into PVs.

Serum levels of sICAM-1 have been elevated in patients suffering from liver metastases. The highest levels of this protein are found in liver metastases. SICAM-1 is produced by different cells of the liver, and it is responsible for numerous pathways that lead to metastasis. LSECs secrete sICAM-1 whenever they are exposed to inflamatory stimuli. This enhances their metastatic ability and promotes expression of adhesion molecules. Moreover human microvascular lung endothelial cells have been proven to secrete sICAM-1.

The expression of ICAM1 is controlled by several transcription factors. The gene expression is controlled by several transcription factors. STAT3 and STAT1p have been identified to increase its activity, while NFKappaB (and 8CREB) regulate its expression. ICAM1 gene expression could be slowed down by microRNA (miR-221). Further research is needed in order to understand how ICAM1 gene activity affects human health.

Recent research has focused on the levels of sICAM-1 in cerebrospinal liquid and postmortem CNS tissues in patients suffering from psychiatric disorders. Both sICAM-1 and the duration of the disorder are key factors in psychiatric diseases. Researchers concluded that these polymorphisms may to affect the communication between the CNS immune system and peripheral immune system.

Although ICAM-1 is not usually considered to be an antigen that is expressed on cells, it is an important regulator of macrophage function in a variety of situations of infection and inflammation. Studies in the early years showed an increase in ICAM-1 expression in human macrophage cell line THP1 and bone Marrow-derived macrophages. It is also stimulated in mice by inflammation. It has also been proven that ICAM-1 expression is related to macrophage the polarization.

Although ICAM-1 was initially thought to be an adhesion molecule with a simple structure however, research has shown that it plays a key role in human rhinovirus (HRV) entry into various types of cells. In light of these studies, scientists are now looking for ways to control the amount of ICAM-1 on cell surfaces and also to control HRV infection. It is crucial to understand that ICAM-1 levels can be increased in human cells in order to reduce the severity and virulence of HRV.