Anti-Calpain 1/CAPN1 Antibody Picoband™

Boster Bio Anti-Calpain 1/CAPN1 Antibody Picoband™ catalog # PB9330. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PB9330
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Calpain 1/CAPN1 Antibody Picoband™

See all Calpain 1 products

SKU/Catalog Number

PB9330

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Calpain 1/CAPN1 Antibody Picoband™ catalog # PB9330. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calpain 1/CAPN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9330)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9330 is reactive to CAPN1 in Human, Mouse, Rat

Applications

PB9330 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CAPN1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P07384

Gene ID

823

Gene Name

CAPN1

Full Name

Calpain-1 catalytic subunit

Weight

81.89kDa

Superfamily

peptidase C2 family

Alternative Names

Calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; Calpain mu-type; calpain, large polypeptide L1; calpain-1 catalytic subunit; Calpain-1 large subunit; CANP 1; CANP; CANPL1EC 3.4.22.52; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; EC 3.4.22; micromolar-calpain; muCANPCANP1; muCL CAPN1 CANP, CANP1, CANPL1, SPG76, muCANP, muCL calpain 1 calpain-1 catalytic subunit|CANP 1|calcium-activated neutral proteinase 1|calpain 1, (mu/I) large subunit|calpain mu-type|calpain, large polypeptide L1|calpain-1 large subunit|cell proliferation-inducing gene 30 protein|cell proliferation-inducing protein 30|micromolar-calpain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CAPN1, check out the CAPN1 Infographic

CAPN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CAPN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9330 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Mo Xg, Chen Qw, Li Xs, Zheng Mm, Ke Dz, Deng W, Li Gq, Jiang J, Wu Zq, Wang L, Wang P, Yang Y, Cao Gy. Microvasc Res. 2011 Mar;81(2):160-8. Doi: 10.1016/J.Mvr.2010.12.004. Epub 2010 Dec 24. Suppression Of Nhe1 By Small Interfering Rna Inhibits Hif...

Chen Hx, Tang Sp, Gao Ft, Xu Jl, Jiang Xp, Cao J, Fu Gb, Sun K, Liu Sz, Shi W. Medicine (Baltimore). 2014 Nov;93(23):E138. Doi: 10.1097/Md.0000000000000138. Fibrosis, Adipogenesis, And Muscle Atrophy In Congenital Muscular Torticollis.

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4 Customer Q&As for Anti-Calpain 1/CAPN1 Antibody Picoband™

Question

We are currently using anti-Calpain 1/CAPN1 antibody PB9330 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

The anti-Calpain 1/CAPN1 antibody (PB9330) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-16

Question

We have been able to see staining in mouse pancreas placenta. Do you have any suggestions? Is anti-Calpain 1/CAPN1 antibody supposed to stain pancreas placenta positively?

Verified Customer

Verified customer

Asked: 2018-08-31

Answer

From what I have seen in literature pancreas placenta does express CAPN1. From what I have seen in Uniprot.org, CAPN1 is expressed in esophagus mucosa, kidney, pancreas placenta, erythroleukemia, liver, among other tissues. Regarding which tissues have CAPN1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pancreas, and Placenta, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-08-31

Question

My team were satisfied with the WB result of your anti-Calpain 1/CAPN1 antibody. However we have seen positive staining in esophagus mucosa cytoplasm using this antibody. Is that expected? Could you tell me where is CAPN1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

According to literature, esophagus mucosa does express CAPN1. Generally CAPN1 expresses in cytoplasm. Regarding which tissues have CAPN1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pancreas, and Placenta, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-07-11

Question

I would like using your anti-Calpain 1/CAPN1 antibody for positive regulation of cell population proliferation studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-04-05

Answer

Thank you for your inquiry. This PB9330 anti-Calpain 1/CAPN1 antibody is tested on rat lung tissue, tissue lysate, mouse lung tissue, a549 whole cell lysate, colo320 whole cell lysate, jurkat whole cell lysate, mammary cancer tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-04-05

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PB9330
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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