Anti-Hu CD83 Purified Monoclonal Antibody

CD83 antibody

Boster Bio Anti-Hu CD83 Purified Monoclonal Antibody (Catalog# M01777). Tested in Flow Cytometry, IHC-F application(s). This antibody reacts with Human, Primate.

Product Info Summary

SKU: M01777
Size: 0.1 mg
Reactive Species: Human, Primate
Host: Mouse
Application: Flow Cytometry, IHC-F

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Product Name

Anti-Hu CD83 Purified Monoclonal Antibody

View all CD83 Antibodies

SKU/Catalog Number

M01777

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD83 Purified Monoclonal Antibody (Catalog# M01777). Tested in Flow Cytometry, IHC-F application(s). This antibody reacts with Human, Primate.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD83 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01777)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

HB15e

Isotype

Mouse IgG1 kappa

Immunogen

Human CD83-transfected Cos cells. The mouse monoclonal antibody HB15e recognizes an extracellular epitope of CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01777 is reactive to CD83 in Human, Primate

Applications

M01777 is guaranteed for Flow Cytometry, IHC-F Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

23.042kDa

Background of CD83

CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 µg/mL, Positive control: peripheral blood-derived dendritic cells.
Immunohistochemistry (frozen sections): acetone fixation.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For CD83 (Source: Uniprot.org, NCBI)

Gene Name

CD83

Full Name

CD83 antigen

Weight

23.042kDa

Alternative Names

B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83 CD83 BL11, HB15 CD83 molecule CD83 antigen|B-cell activation protein|CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)|cell surface protein HB15|cell-surface glycoprotein|hCD83

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD83, check out the CD83 Infographic

CD83 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD83: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M01777

0.1 mg

$298

Free Secondary Antibody

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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