Anti-Hu CD83 Purified Monoclonal Antibody

Product Name

Anti-Hu CD83 Purified Monoclonal Antibody

View all CD83 Antibodies

SKU/Catalog Number

M01777

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD83 Purified Monoclonal Antibody (Catalog# M01777). Tested in Flow Cytometry, IHC-F application(s). This antibody reacts with Human, Primate.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD83 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01777)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

HB15e

Isotype

Mouse IgG1 kappa

Immunogen

Human CD83-transfected Cos cells. The mouse monoclonal antibody HB15e recognizes an extracellular epitope of CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.

Reactive Species

M01777 is reactive to CD83 in Human, Primate

Observed Molecular Weight

42 kDa

Calculated molecular weight

23.0 kDa

Background of CD83

CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01777 is guaranteed for Flow Cytometry, IHC-F Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 µg/mL, Positive control: peripheral blood-derived dendritic cells.
Immunohistochemistry (frozen sections): acetone fixation.

Validation Images & Assay Conditions

See full target information CD83

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