Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated

Boster Bio Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated catalog # FC04535-FITC. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: FC04535-FITC
Size: 100 Tests, 200 Tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated

See all SSEA-1 products

SKU/Catalog Number

FC04535-FITC

Size

100 Tests, 200 Tests

Form

Liquid

Description

Boster Bio Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated catalog # FC04535-FITC. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at 4°C for one year. Do not freeze.

Cite This Product

Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC04535-FITC)

Host

Mouse

Contents

PBS with 0.09% NaN3 and 0.2% (w/v) BSA

Clonality

Monoclonal

Clone Number

FUT4/815

Isotype

IgM,k

Immunogen

Purified human neutrophils

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC04535-FITC is reactive to FUT4 in Human

Applications

FC04535-FITC is guaranteed for Flow Cytometry Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 5μl is enough to stain 1 million PBMCs or 100μl whole blood sample

Validation Images & Assay Conditions

Gene/Protein Information For FUT4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P22083

Gene ID

2526

Gene Name

FUT4

Full Name

Alpha-(1,3)-fucosyltransferase 4

Weight

59.084kDa

Superfamily

glycosyltransferase 10 family

Alternative Names

alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1 FUT4 CD15, ELFT, FCT3A, FUC-TIV, FUTIV, LeX, SSEA-1 fucosyltransferase 4 alpha-(1,3)-fucosyltransferase 4|ELAM ligand fucosyltransferase|ELAM-1 ligand fucosyltransferase|Lewis X|alpha (1,3) fucosyltransferase, myeloid-specific|fucT-IV|fucosyltransferase IV|galactoside 3-L-fucosyltransferase|stage-specific embryonic antigen 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FUT4, check out the FUT4 Infographic

FUT4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FUT4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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11 Customer Q&As for Anti-human CD15 FUT4 Monoclonal Antibody FITC Conjugated, Flow Validated

Question

I see that the anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-19

Question

Is a blocking peptide available for product anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated (FC04535-FITC)?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

We do provide the blocking peptide for product anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated (FC04535-FITC). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-31

Question

See below the WB image, lot number and protocol we used for testis using anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-09

Question

Can you help my question with product FC04535-FITC, anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-07

Question

Is this FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated reactive to the isotypes of FUT4?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

The immunogen of FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated is Purified human neutrophils. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-01

Question

I am interested in to test anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC on human testis for research purposes, then I may be interested in using anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

G. Williams

Verified customer

Asked: 2019-09-23

Answer

The products we sell, including anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-23

Question

Will FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

You can see on the product datasheet, FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-26

Question

Do you have a BSA free version of anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC available?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-22

Question

Will anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC work for Flow Cytometry with testis?

Z. Carter

Verified customer

Asked: 2019-01-28

Answer

According to the expression profile of testis, FUT4 is highly expressed in testis. So, it is likely that anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC will work for Flow Cytometry with testis.

Boster Scientific Support

Answered: 2019-01-28

Question

I was wanting to use your anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated for Flow Cytometry for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?

Verified Customer

Verified customer

Asked: 2019-01-17

Answer

It shows on the product datasheet, FC04535-FITC anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-17

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-human CD15 Monoclonal antibody FITC Conjugated, Flow Validated FC04535-FITC. Let me know if you need anything else.

K. Patel

Verified customer

Asked: 2017-09-26

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-26

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
FC04535-FITC
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$235.00

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