Anti-CEACAM5 Antibody Picoband™

CEACAM5/CD66e antibody

Boster Bio Anti-CEACAM5 Antibody Picoband™ catalog # A00356. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00356
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-CEACAM5 Antibody Picoband™

View all CEACAM5/CD66e Antibodies

SKU/Catalog Number

A00356

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CEACAM5 Antibody Picoband™ catalog # A00356. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CEACAM5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00356)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human CEACAM5 recombinant protein (Position: K35-N650).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00356 is reactive to CEACAM5 in Human, Mouse, Rat

Applications

A00356 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of CEACAM5/CD66e

CEA (Carcinoembryonic antigen) is a glycoprotein involved in cell adhesion. It is normally produced during fetal development, but the production of CEA stops before birth. Therefore, it is not usually present in the blood of healthy adults, although levels are raised in heavy smokers. CEA is a glycosyl phosphatidyl inositol (GPI)-cell surface anchored glycoprotein whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and E-selectin ligands, which may be critical to the metastatic dissemination of colon carcinoma cells. CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

"Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human
"

Validation Images & Assay Conditions

Gene/Protein Information For CEACAM5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P06731

Gene ID

1048

Gene Name

CEACAM5

Full Name

Carcinoembryonic antigen-related cell adhesion molecule 5

Weight

76.795kDa

Superfamily

immunoglobulin superfamily

Alternative Names

Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100 CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic antigen-related cell adhesion molecule 5|carcinoembryonic antigen related cell adhesion molecule 5|meconium antigen 100

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CEACAM5, check out the CEACAM5 Infographic

CEACAM5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CEACAM5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00356 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

An aptasensor for carcinoembryonic antigen based on upconversion fluorescence resonance energy transfer

Fluorescence resonance energy transfer biosensor between upconverting nanoparticles and palladium nanoparticles for ultrasensitive CEA detection

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5 Customer Q&As for Anti-CEACAM5 Antibody Picoband™

Question

We have been able to see staining in human colonic mucosa. Any tips? Is anti-CEACAM5 antibody supposed to stain colonic mucosa positively?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

From literature colonic mucosa does express CEACAM5. From Uniprot.org, CEACAM5 is expressed in colonic mucosa, saliva, liver, among other tissues. Regarding which tissues have CEACAM5 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Saliva, Pubmed ID: 16740002

Boster Scientific Support

Answered: 2019-08-19

Question

We have tried in the past anti-CEACAM5 antibody for ELISA on saliva in a previous project. I am using human, and We are going to use the antibody for WB next. I would like examining saliva as well as colonic mucosa in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-02-11

Answer

I have checked the website and datasheets of our anti-CEACAM5 antibody and I see that A00356 has been tested on human in both ELISA and WB. Thus A00356 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-02-11

Question

We were well pleased with the WB result of your anti-CEACAM5 antibody. However we have seen positive staining in saliva cell membrane using this antibody. Is that expected? Could you tell me where is CEACAM5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-02-01

Answer

According to literature, saliva does express CEACAM5. Generally CEACAM5 expresses in cell membrane. Regarding which tissues have CEACAM5 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Saliva, Pubmed ID: 16740002

Boster Scientific Support

Answered: 2019-02-01

Question

I am looking for using your anti-CEACAM5 antibody for heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules studies. Has this antibody been tested with western blotting on rat intestine tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-07-13

Answer

Thanks for your inquiry. This A00356 anti-CEACAM5 antibody is validated on human sw620 whole cell lysates, colon cancer tissue, intestinal cancer tissue, lung cancer tissue, mouse intestine tissue, rat intestine tissue. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-07-13

Question

We are currently using anti-CEACAM5 antibody A00356 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-02-05

Answer

The anti-CEACAM5 antibody (A00356) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-02-05

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00356

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A00356
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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