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- Table of Contents
CEACAM5 antibody (A00356) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356).
CEACAM5 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
CEACAM5 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-CEACAM5 Antibody Picoband®
CEACAM5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
CEACAM5 antibody was positively detected by WB in:
human SW620 whole cell, human Caco-2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CEACAM5 antibody was positively detected by IHC in:
human colon cancer tissue, human colon cancer tissue, human intestinal cancer tissue, human lung cancer tissue, human lung cancer tissue, human lung cancer tissue, mouse intestine tissue, rat intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
"Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
ELISA
0.1-0.5μg/ml
-
"
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Product Information
Product Name
Anti-CEACAM5 Antibody Picoband®
View all CEACAM5 Antibodies
View all CEACAM5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CEACAM5 recombinant protein (Position: K35-N650).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130-200 kDa
Calculated Molecular Weight
76.8 kDa
Background of CEACAM5
CEA (Carcinoembryonic antigen) is a glycoprotein involved in cell adhesion. It is normally produced during fetal development, but the production of CEA stops before birth. Therefore, it is not usually present in the blood of healthy adults, although levels are raised in heavy smokers. CEA is a glycosyl phosphatidyl inositol (GPI)-cell surface anchored glycoprotein whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and E-selectin ligands, which may be critical to the metastatic dissemination of colon carcinoma cells. CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed.
Cite This Product
Anti-CEACAM5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00356)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
79%
Moderate Homology
Mouse
34%
Low homology
Rabbit
34%
Low homology
Rat
33%
Low homology
Zebrafish
29%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CEACAM5 antibody
Immunohistochemistry (IHC) protocol for CEACAM5 antibody
Publications
Application:
Species:
Band-Edge Effect-Induced Electrochemiluminescence Signal Amplification Based on Inverse Opal Photonic Crystals for Ultrasensitive Detection of Carcinoembryonic Antigen
Published: 2022-06-24
Journal: ANALYTICAL CHEMISTRY
Application: IA
Impact Factor: 8.01
Species: immunosensor
PMID: 35749110
An aptasensor for carcinoembryonic antigen based on upconversion fluorescence resonance energy transfer
Published: 2014-10-02
Journal: SENSORS AND ACTUATORS B-CHEMICAL
Impact Factor: 3.84
Review
Have you used Anti-CEACAM5 Antibody Picoband®?
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1 Review For Anti-CEACAM5 Antibody Picoband®
In IHC of human skin cancer sections, the CEACAM5 antibody (A00356) clearly distinguished melanoma from other skin cancers, showing specific staining with low background.
| SKU | M12477-15 |
|---|---|
| Application | Immunohistochemistry |
| Sample | normal and preterm human placentas |
| Sample Processing Description | Clinically collected normal and preterm placentas were sectioned longitudinally (with amnion and chorion visible) and prepared as paraffin-embedded sections. |
| Other Reagents | Tris-EDTA Antigen Retrieval Solution, DAB Chromogenic Solution |
| Primary Antibody | Histone H3 (acetyl K27) Rabbit Monoclonal Antibody |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | Polymer anti-rabbit IgG-HRP IHC detection kit |
| Secondary Incubation | 45 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | Histone H3 (acetyl K27) is the acetylated form of histone H3 at lysine 27 and serves as a sensitive indicator of epigenetic abnormalities in placental cells; IHC results showed higher expression in preterm placentas than in normal placentas. |
Miao Zhang, SINH
Verified customer
Submitted 2026-02-06
Product Promise
We promise Anti-CEACAM5 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00356
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026