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Product Info Summary
| SKU: | M11385-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120
SKU/Catalog Number
M11385-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 (Catalog # M11385-2). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11385-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM120
Isotype
Rabbit IgG
Immunogen
Peptide corresponding to the hinge region of Human IgG4
Cross-reactivity
Reacts to the heavy chain of human IgG4, RM120 does not cross-react to any other IgG subclasses (IgG1, IgG2, IgG3), and shows no cross-reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M11385-2 is reactive to IGHG4 in Human
Calculated molecular weight
35.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11385-2 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Recommend Dilution
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
WB: 0.5 ug/mL – 2 ug/mL
Flow Cytometry (FC): 1ug/mL-4ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of human, mouse, rat, and goat IgG shows RM120 reacted to human IgG4 both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced). No cross reactivity with other isotype of human IgG, and mouse, rat, or goat IgG.
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IHC result
Immunohistochemistry of human lymphoid tissue using anti-human IgG4 antibody RM120.
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ELISA result showing specificity
Detection of human IgG4 in monkey serum. Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains ( or ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.
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ELISA result showing specificity
Sandwich ELISA using RM120 as the capture antibody, and Biotinylated anti-human light chains ( + ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
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ELISA result showing specificity
A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
ELISA showing RM120 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM120 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
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IHC result
Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG4 antibody RM120 at a 1:50,000 dilution.
Specific Publications For Anti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 (M11385-2)
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