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Product Info Summary
| SKU: | M11385-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M11385-1
BM4295 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody catalog # M11385-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11385-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DCF-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IgG4
Reactive Species
M11385-1 is reactive to IGHG4 in Human
Observed Molecular Weight
52 kDa
Calculated molecular weight
35.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11385-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human hepatocellular carcinoma tumor tissue, human hepatocellular carcinoma paracancerous tissue
IHC: human stomach carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of IGHG4 using anti-IGHG4 antibody (M11385-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGHG4 antigen affinity purified monoclonal antibody (M11385-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IGHG4 at approximately 52 kDa. The expected band size for IGHG4 is at 52 kDa.
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Immunohistochemical analysis of paraffin-embedded human stomach carcinoma, using human IgG4 Antibody .
Specific Publications For Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody (M11385-1)
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Customer Q&As
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6 Customer Q&As for Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-IgG4 Rabbit Monoclonal antibody (M11385-1)?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We do provide the blocking peptide for product anti-IgG4 Rabbit Monoclonal antibody (M11385-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-25
Question
Is there a BSA free version of anti-IgG4 Rabbit Monoclonal antibody M11385-1 available?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IgG4 Rabbit Monoclonal antibody M11385-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-11
Question
Can you help my question with product M11385-1, anti-IgG4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M11385-1 anti-IgG4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-09
Question
We need to test anti-IgG4 Rabbit Monoclonal antibody M11385-1 on human lymph node for research purposes, then I may be interested in using anti-IgG4 Rabbit Monoclonal antibody M11385-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
The products we sell, including anti-IgG4 Rabbit Monoclonal antibody M11385-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-28
Question
Does M11385-1 anti-IgG4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-19
Answer
As indicated on the product datasheet, M11385-1 anti-IgG4 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-19
Question
Will anti-IgG4 Rabbit Monoclonal antibody M11385-1 work on monkey WB with liver?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
Our lab technicians have not validated anti-IgG4 Rabbit Monoclonal antibody M11385-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-IgG4 Rabbit Monoclonal antibody M11385-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-24
