Anti-Human IL-17F Biotin Conjugated Monoclonal Antibody

Product Name

Anti-Human IL-17F Biotin Conjugated Monoclonal Antibody

View all IL17F Antibodies

SKU/Catalog Number

B02062-1

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Human IL-17F Biotin Conjugated Monoclonal Antibody (Catalog # B02062-1). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-Human IL-17F Biotin Conjugated Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # B02062-1)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

Clone: 4H1629.1 IgG1

Isotype

IgG1

Immunogen

Anti-IL-17F (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17F produced in E.coli followed by hybridoma development.

Reactive Species

B02062-1 is reactive to IL17F in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

18.0 kDa

Background of IL17F

Anti-L-17F recognizes IL-17F (also known as Cytokine ML-1 or Interleukin-24). IL-17F is produced and secreted by CD8+ T cells, NK cells, NKT cells and LTi cells. The main functions of IL-17F are neutrophil recruitment and immunity to extracellular pathogen. More importantly, IL-17F drives inflammation and auto-immunity. IL-17A and IL-17F are by far the best characterized cytokines of the IL-17 cytokine family. IL-17F dimerizes in a parallel fashion similar to nerve growth factor and other neutrophins. Its dimerization is critical to fulfill its activity. When secreted by activated T cells, IL-17F can stimulate the production of other cytokines such as IL-6, IL-8 granulocyte colony-stimulating factor and, can stimulate cartilage matrix turnover. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and Inflammatory pathologies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

B02062-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000-1:50,000
Fluorochrome Protein Value: 10-20
IHC: 5µg/mL
WB: 0.5 mg/mL
This purified antibody has been tested for use in IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Validation Images & Assay Conditions

See full target information IL17F

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