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Product Info Summary
| SKU: | M02062 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse |
| Host: | Mouse |
| Application: | IHC-P |
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Product info
Product Name
Anti- IL-17F Monoclonal Antibody
SKU/Catalog Number
M02062
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- IL-17F Monoclonal Antibody catalog # M02062. Tested in IHC-P applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IL-17F Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02062)
Host
Mouse
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFIC-9
Isotype
Mouse IgG1, κ
Immunogen
Mouse IL-17F
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02062 is reactive to Il17f in Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
17.9 kDa
Background of Il17f
Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02062 is guaranteed for IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
Positive Control
IHC: mouse spleen tissue
Validation Images & Assay Conditions
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IHC analysis of IL-17F using anti-IL-17F antibody (M02062).
IL-17F was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-IL-17F Antibody (M02062) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Specific Publications For Anti- IL-17F Monoclonal Antibody (M02062)
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