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Il17f antibody (M02062) performs well in IHC-P and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of IL-17F using anti-IL-17F antibody (M02062).
IL-17F was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-IL-17F Antibody (M02062) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
IL-17F was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-IL-17F Antibody (M02062) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Anti- IL-17F Monoclonal Antibody
Il17f antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Mouse | IHC-P |
Tested Applications
Il17f antibody was positively detected by IHC in:
mouse spleen tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti- IL-17F Monoclonal Antibody
View all IL17F Antibodies
View all IL17F Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFIC-9
Isotype
Mouse IgG1, κ
Immunogen
Mouse IL-17F
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μ/ml.
Observed Molecular Weight
42 kDa
Calculated Molecular Weight
17.9 kDa
Background of Il17f
Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has
been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.
Cite This Product
Anti- IL-17F Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02062)
Protocols
Immunohistochemistry (IHC) protocol for Il17f antibody
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Product Promise
We promise Anti- IL-17F Monoclonal Antibody performs excellently in relevant applications for IHC-P. If it doesn't perform as promised, we will refund the product. Learn more
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M02062
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026