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Product Info Summary
| SKU: | A00963-1 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL-22/IL22 Antibody Picoband™
SKU/Catalog Number
A00963-1
Size
100ug/vial
Form
Lyophilized
Description
Boster Bio Anti-IL-22/IL22 Antibody Picoband™ catalog # A00963-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-22/IL22 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00963-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Immunogen
E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22.
Cross Reactivity
No cross reactivity with other proteins.
Reactive Species
A00963-1 is reactive to IL22 in Human, Mouse, Rat
Applications
A00963-1 is guaranteed for ELISA, WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Human,
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat,
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IL-22 using anti-IL-22 antibody (A00963-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysate,
Lane 2: mouse HEPA1-6 whole cell lysate,
Lane 3: rat PC-12 whole cell lysate,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-22 antigen affinity purified polyclonal antibody (Catalog # A00963-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-22 at approximately 22KD. The expected band size for IL-22 is at 20KD.
Protein Target Info & Infographic
Gene/Protein Information For IL22 (Source: Uniprot.Org, NCBI)
Uniprot ID
Q9GZX6
Gene ID
50616
Gene Name
IL22
Full Name
Interleukin-22
Weight
20.011kDa
Superfamily
IL-10 family
Alternative Names
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18 IL22 IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18 interleukin 22 interleukin-22|IL-10-related T-cell-derived inducible factor|cytokine Zcyto18
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL22, check out the IL22 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-IL-22/IL22 Antibody Picoband™ (A00963-1)
No publications found for A00963-1
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Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
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- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
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- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Anti-IL-22/IL22 Antibody Picoband™
Question
I was wanting to use your anti-IL-22/IL22 antibody for WB for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
You can see on the product datasheet, A00963-1 anti-IL-22/IL22 antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-18
Question
I would like to test anti-IL-22/IL22 antibody A00963-1 on human blood for research purposes, then I may be interested in using anti-IL-22/IL22 antibody A00963-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
The products we sell, including anti-IL-22/IL22 antibody A00963-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-21
Question
We are currently using anti-IL-22/IL22 antibody A00963-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The anti-IL-22/IL22 antibody (A00963-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-IL-22/IL22 antibody A00963-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-08
Question
Please see the WB image, lot number and protocol we used for blood using anti-IL-22/IL22 antibody A00963-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-15
Question
Is there a BSA free version of anti-IL-22/IL22 antibody A00963-1 available?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-22/IL22 antibody A00963-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-20
Question
I was wanting to use using your anti-IL-22/IL22 antibody for cytokine-mediated signaling pathway studies. Has this antibody been tested with western blotting on human hepg2 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We appreciate your inquiry. This A00963-1 anti-IL-22/IL22 antibody is validated on human hepg2 whole cell lysate. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-16
Question
I see that the anti-IL-22/IL22 antibody A00963-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-22
Question
Is a blocking peptide available for product anti-IL-22/IL22 antibody (A00963-1)?
S. Moore
Verified customer
Asked: 2019-05-23
Answer
We do provide the blocking peptide for product anti-IL-22/IL22 antibody (A00963-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-23
Question
We have observed staining in mouse zone of skin. Any tips? Is anti-IL-22/IL22 antibody supposed to stain zone of skin positively?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
According to literature zone of skin does express IL22. According to Uniprot.org, IL22 is expressed in zone of skin, blood, among other tissues. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-17
Question
My boss were content with the WB result of your anti-IL-22/IL22 antibody. However we have seen positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL22 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
According to literature, blood does express IL22. Generally IL22 expresses in secreted. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-12-14
Question
Does A00963-1 anti-IL-22/IL22 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Walker
Verified customer
Asked: 2018-11-22
Answer
You can see on the product datasheet, A00963-1 anti-IL-22/IL22 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-22
Question
My question regarding product A00963-1, anti-IL-22/IL22 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00963-1 anti-IL-22/IL22 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-07
Question
We purchased anti-IL-22/IL22 antibody for ELISA on blood a few years ago. I am using human, and We want to use the antibody for WB next. I was wanting to use examining blood as well as zone of skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-04-11
Answer
I took a look at the website and datasheets of our anti-IL-22/IL22 antibody and I see that A00963-1 has been validated on human in both ELISA and WB. Thus A00963-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-11
Question
Would anti-IL-22/IL22 antibody A00963-1 work for WB with blood?
C. Jackson
Verified customer
Asked: 2016-06-09
Answer
According to the expression profile of blood, IL22 is highly expressed in blood. So, it is likely that anti-IL-22/IL22 antibody A00963-1 will work for WB with blood.
Boster Scientific Support
Answered: 2016-06-09
Question
Is this A00963-1 anti-IL-22/IL22 antibody reactive to the isotypes of IL22?
O. Wu
Verified customer
Asked: 2013-07-02
Answer
The immunogen of A00963-1 anti-IL-22/IL22 antibody is E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-07-02
