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Product Info Summary
| SKU: | RP1024 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Growth Hormone/GH1 Antibody Picoband®
SKU/Catalog Number
RP1024
BA14245 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Growth Hormone/GH1 Antibody catalog # RP1024. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Growth Hormone/GH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1024)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse GH recombinant protein (Position: F27-F216).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1024 is reactive to Gh1 in Mouse
Observed Molecular Weight
22 kDa
Calculated molecular weight
24.7 kDa
Background of Gh1
Growth Hormone (GH) is mapped to 17q22-q24. Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1024 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Anti-mouse Growth Hormone antibody, RP1024, Western blotting
Lane 1: Recombinant Mouse GH Protein 10ng
Lane 2: Recombinant Mouse GH Protein 5ng
Lane 3: Recombinant Mouse GH Protein 2
Specific Publications For Anti-Growth Hormone/GH1 Antibody Picoband® (RP1024)
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Customer Q&As
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6 Customer Q&As for Anti-Growth Hormone/GH1 Antibody Picoband®
Question
I was wanting to use your anti-Growth Hormone/GH1 antibody for WB for mouse adenohypophysis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse adenohypophysis identification?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
You can see on the product datasheet, RP1024 anti-Growth Hormone/GH1 antibody has been tested for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse adenohypophysis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-Growth Hormone/GH1 antibody RP1024 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The anti-Growth Hormone/GH1 antibody (RP1024) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-Growth Hormone/GH1 antibody RP1024. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-03
Question
I have attached the WB image, lot number and protocol we used for adenohypophysis using anti-Growth Hormone/GH1 antibody RP1024. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-18
Question
My question regards to test anti-Growth Hormone/GH1 antibody RP1024 on mouse adenohypophysis for research purposes, then I may be interested in using anti-Growth Hormone/GH1 antibody RP1024 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
The products we sell, including anti-Growth Hormone/GH1 antibody RP1024, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-06
Question
Does RP1024 anti-Growth Hormone/GH1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Baker
Verified customer
Asked: 2013-10-14
Answer
As indicated on the product datasheet, RP1024 anti-Growth Hormone/GH1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-10-14
