Anti-Growth Hormone/GH1 Antibody

Growth Hormone antibody

Boster Bio Anti-Growth Hormone/GH1 Antibody catalog # RP1024. Tested in WB applications. This antibody reacts with Mouse.

Product Info Summary

SKU: RP1024
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: WB

Product Name

Anti-Growth Hormone/GH1 Antibody

View all Growth Hormone Antibodies

SKU/Catalog Number

RP1024

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Growth Hormone/GH1 Antibody catalog # RP1024. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Growth Hormone/GH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1024)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse GH recombinant protein (Position: F27-F216).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1024 is reactive to GH1 in Mouse

Applications

RP1024 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

24.847kDa

Background of Growth Hormone

Growth Hormone (GH) is mapped to 17q22-q24. Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For GH1 (Source: Uniprot.org, NCBI)

Gene Name

GH1

Full Name

Somatotropin

Weight

24.847kDa

Superfamily

somatotropin/prolactin family

Alternative Names

GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin GH1 GH, GH-N, GHB5, GHN, IGHD1A, IGHD1B, IGHD2, hGH-N growth hormone 1 somatotropin|growth hormone B5|pituitary growth hormone

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GH1, check out the GH1 Infographic

GH1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Growth Hormone/GH1 Antibody

Question

I was wanting to use your anti-Growth Hormone/GH1 antibody for WB for mouse adenohypophysis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse adenohypophysis identification?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

You can see on the product datasheet, RP1024 anti-Growth Hormone/GH1 antibody has been tested for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse adenohypophysis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-20

Question

We are currently using anti-Growth Hormone/GH1 antibody RP1024 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

The anti-Growth Hormone/GH1 antibody (RP1024) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-09

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-Growth Hormone/GH1 antibody RP1024. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-03

Question

I have attached the WB image, lot number and protocol we used for adenohypophysis using anti-Growth Hormone/GH1 antibody RP1024. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-12-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-18

Question

My question regards to test anti-Growth Hormone/GH1 antibody RP1024 on mouse adenohypophysis for research purposes, then I may be interested in using anti-Growth Hormone/GH1 antibody RP1024 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-08-06

Answer

The products we sell, including anti-Growth Hormone/GH1 antibody RP1024, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-06

Question

Does RP1024 anti-Growth Hormone/GH1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Baker

Verified customer

Asked: 2013-10-14

Answer

As indicated on the product datasheet, RP1024 anti-Growth Hormone/GH1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-10-14

Order DetailsPrice
RP1024

100μg

$370
RP1024-10ug

10μg sample (liquid)

$99
RP1024-Biotin

100 μg Biotin conjugated

$570
RP1024-Cy3

100 μg Cy3 conjugated

$570
RP1024-Dylight488

100 μg Dylight488 conjugated

$570
RP1024-Dylight550

100 μg Dylight550 conjugated

$570
RP1024-Dylight594

100 μg Dylight594 conjugated

$570
RP1024-FITC

100 μg FITC conjugated

$570
RP1024-HRP

100 μg HRP conjugated

$570
RP1024-APC

100 μg APC conjugated

$670
RP1024-PE

100 μg PE conjugated

$670
RP1024-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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