ICAM1 antibody (A00171) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 38 Publications
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Anti-ICAM1 Antibody Picoband®

ICAM1 antibody

Reactive SpeciesHostApplication
Human, Mouse, RatRabbitELISA, Flow Cytometry, IF, IHC, ICC, WB

SKU: A00171

Size: 100 μg

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Price:
$370/100 μg

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Tested Applications

ICAM1 antibody was positively detected by WB in:

rat spleen tissue, rat thymus tissue, rat RH35 cell, mouse spleen tissue, mouse thymus tissue, mouse HEPA1-6 cell, mouse heart tissue, human Raji whole cell, human K562 whole cell, human HepG2 whole cell, human 293T whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ICAM1 antibody was positively detected by IHC in:

human lung cancer tissue, human bladder urothelial carcinoma tissue, human colorectal adenocarcinoma tissue, human placenta tissue, human spleen tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

ICAM1 antibody was positively detected by ICC/IF in:

A431 cell

ICAM1 antibody was positively detected by FC in:

PC-3 cell

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
0.1-0.5μg/ml

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Reactive Data

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WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Tested
Tested
Recommend
Recommend

Product Information

Product Name
Anti-ICAM1 Antibody Picoband®
View all ICAM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ICAM1 recombinant protein (Position: Q28-R268).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa
Calculated Molecular Weight

57.8 kDa

Background of ICAM1
CD54, also known as ICAM-1. Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. ICAM-1 is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. This protein is the major human rhinovirus receptor. The ICAM1 gene is mapped to human chromosome 19. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).
Cite This Product
Anti-ICAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00171)

Protocols

Western Blot protocol for ICAM1 antibody
Immunohistochemistry (IHC) protocol for ICAM1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ICAM1 antibody
Flow Cytometry (FC) protocol for ICAM1 antibody

Publications

Application:
Species:
Association of 17β-estradiol and methylprednisolone protects female kidneys from brain death induced inflammation
Published: 2025-10-24
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC
Impact Factor: 4.70
Species: Rat
Endothelial Gasdermin D Induces Mitochondrial Damage and Activates the STING Pathway in Lipopolysaccharide-Accelerated Atherosclerosis
Published: 2025-09-29
Journal: ANTIOXIDANTS & REDOX SIGNALING
Application: WB
Impact Factor: 6.10
Species: Mouse,Human
PMID: 41017337
Endothelial HMGB1-AIM2 axis worsens myocardial ischemia—reperfusion injury by regulating endothelial pyroptosis
Published: 2025-08-14
Journal: APOPTOSIS
Application: IF
Impact Factor: 8.10
Species: Mouse
PMID: 40813539
The Protective Role of Intermedin in Contrast-Induced Acute Kidney Injury: Enhancing Peritubular Capillary Endothelial Cell Adhesion and Integrity Through the cAMP/Rac1 Pathway
Published: 2024-10-16
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IF
Impact Factor: 4.90
Species: Rat
PMID: 39456892
STAT3 blockade ameliorates LPS-induced kidney injury through macrophage-driven inflammation
Published: 2024-10-04
Journal: Cell Communication and Signaling
Application: WB,IHC
Impact Factor: 8.20
Species: Mouse
PMID: 39367511
Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm
Published: 2024-05-13
Journal: JOURNAL OF BIOMEDICAL SCIENCE
Application: IF
Impact Factor: 9.00
Species: Human
PMID: 38741091
17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats
Published: 2024-05-03
Journal: Frontiers in Immunology
Application: IHC
Impact Factor: 5.70
Species: Rat
PMID: 38765005
Differential vascular endothelial cell toxicity of established and novel BCR-ABL tyrosine kinase inhibitors
Published: 2023-11-20
Journal: PLoS One
Application: WB
Impact Factor: 3.70
Species: Human
PMID: 37983208
GSDME in Endothelial Cells: Inducing Vascular Inflammation and Atherosclerosis via Mitochondrial Damage and STING Pathway Activation
Published: 2023-09-20
Journal: Biomedicines
Application: WB
Impact Factor: 4.70
Species: Mouse,Human
PMID: 37761020
Evaluation of the therapeutic effects of oestradiol on the systemic inflammatory response and on lung injury caused by the occlusion of the proximal descending aorta in male rats
Published: 2023-07-06
Journal: EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
Application: IHC
Impact Factor: 3.40
Species: Rat
PMID: 37410160
“Cocktail” anti-tumor strategy through enhanced antigen exposure with photothermal/chemodynamic therapy
Published: 2022-12-23
Journal: CHEMICAL ENGINEERING JOURNAL
Application: WB,ICC
Impact Factor: 16.74
Species: Mouse
DNA from macrophages induces fibrosis and vasculopathy through POLR3A/STING/type I interferon axis in systemic sclerosis
Published: 2022-06-10
Journal: RHEUMATOLOGY
Application: WB, IHC
Impact Factor: 7.05
Species: Human, Mouse
PMID: 35686918
Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes
Published: 2022-06-07
Journal: Frontiers in Bioengineering and Biotechnology
Application: FC
Impact Factor: 6.06
Species: Rat
PMID: 35747494
Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy
Published: 2022-02-18
Journal: Acta Cirurgica Brasileira
Application: IHC
Impact Factor: 1.56
Species: Mouse
PMID: 35195181
Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy
Published: 2021-02-10
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 6.95
Species: Rat
PMID: 33581205
The Increased Endogenous Sulfur Dioxide Acts as a Compensatory Mechanism for the Downregulated Endogenous Hydrogen Sulfide Pathway in the Endothelial Cell Inflammation
Published: 2018-04-30
Journal: Frontiers in Immunology
Application: WB
Impact Factor: 5.51
Species: Rat,Human
PMID: 29760703
Oxidized low-density lipoprotein-induced microparticles promote endothelial monocyte adhesion via intercellular adhesion molecule 1
Published: 2017-11-07
Journal: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
Application: IHC
Impact Factor: 3.60
Species: Mouse
PMID: 28814403
Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs
Published: 2017-01-11
Journal: BMC Complementary and Alternative Medicine
Application: IF
Impact Factor: 2.29
Species: Rat
PMID: 28077102
Perivascular adipose tissue alleviates inflammatory factors and stenosis in diabetic blood vessels
Published: 2016-09-21
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.37
Species: Rat,Human
PMID: 27664706
The protective effect of melatonin on smoke-induced vascular injury in rats and humans: a randomized controlled trial
Published: 2015-12-17
Journal: JOURNAL OF PINEAL RESEARCH
Application: WB
Impact Factor: 9.60
Species: Rat
PMID: 26681403
Anti-inflammatory and antioxidant effects of curcumin on acute lung injury in a rodent model of intestinal ischemia reperfusion by inhibiting the pathway of NF-Kb
Published: 2015-04-01
Journal: International Journal of Clinical and Experimental Pathology
Application: IHC
Impact Factor: 1.89
Species: Rat
PMID: 26097529
Retinoic Acid Promotes Interleukin-4 Plasmid-Dimethylsulfoxide Topical Transdermal Delivery for Treatment of Psoriasis
Published: 2015-03-24
Journal: Annals of Dermatology
Application: IHC
Impact Factor: 1.39
Species: Mouse
PMID: 25834349
Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury
Published: 2014-09-24
Journal: JOURNAL OF PINEAL RESEARCH
Application: WB
Impact Factor: 7.81
Species: Rat
PMID: 25251422
Accumulation of FLT3+ CD11c+ dendritic cells in psoriatic lesions and the anti-psoriatic effect of a selective FLT3 inhibitor
Published: 2014-06-04
Journal: IMMUNOLOGIC RESEARCH
Application: IHC
Impact Factor: 3.53
Species: Human,Mouse
PMID: 24895100
Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats
Published: 2014-03-29
Journal: FOOD AND CHEMICAL TOXICOLOGY
Application: WB
Impact Factor: 2.61
Species: Rat
PMID: 24685824
Glycyrrhizin protects against porcine endotoxemia through modulation of systemic inflammatory response
Published: 2013-03-11
Journal: CRITICAL CARE
Application: WB
Impact Factor: 4.72
Species: Pig
PMID: 23497622
Protective effect and mechanism of sodium tanshinone II A sulfonate on microcirculatory disturbance of small intestine in rats with sepsis
Published: 2011-08-07
Journal: Journal of Huazhong University of Science and Technology-Medical Sciences
Application: WB,IHC
Impact Factor: 0.41
Species: Rat
PMID: 21823002
Curcumin protects mice against concanavalin A-induced hepatitis by inhibiting intrahepatic intercellular adhesion molecule-1 (ICAM-1) and CXCL10 expression
Published: 2011-06-22
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: IHC
Impact Factor: 2.17
Species: Mouse
PMID: 21695461
Calycosin protects HUVECs from advanced glycation end products-induced macrophage infiltration
Published: 2011-06-06
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: ICC,IF
Impact Factor: 2.47
Species: Human,Mouse
PMID: 21669275
Blockade of PKC-beta protects HUVEC from advanced glycation end products induced inflammation
Published: 2010-09-27
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IHC
Impact Factor: 2.21
Species: Human
PMID: 20875828
Protective effect of carnosol on lung injury induced by intestinal ischemia/reperfusion
Published: 2010-08-26
Journal: SURGERY TODAY
Application: IHC
Impact Factor: 1.05
Species: Rat
PMID: 20740350
Gene therapy for psoriasis in the K14-VEGF transgenic mouse model by topical transdermal delivery of interleukin-4 using ultradeformable cationic liposome
Published: 2010-05-14
Journal: JOURNAL OF GENE MEDICINE
Application: IHC
Impact Factor: 2.97
Species: Mouse
PMID: 20527041
Protective effect of JBP485 on concanavalin A-induced liver injury in mice
Published: 2010-01-08
Journal: JOURNAL OF PHARMACY AND PHARMACOLOGY
Application: IHC
Impact Factor: 1.74
Species: Mouse
PMID: 19505367
A novel conjugate of low-molecular-weight heparin and Cu,Zn-superoxide dismutase: Study on its mechanism in preventing brain reperfusion injury after ischemia in gerbils
Published: 2009-01-18
Journal: BRAIN RESEARCH
Application: IHC
Impact Factor: 2.49
Species: Mongolian Gerbil
PMID: 19401175
Laennec Protects Murine from Concanavalin A-Induced Liver Injury through Inhibition of Inflammatory Reactions and Hepatocyte Apoptosis
Published: 2008-11-01
Journal: BIOLOGICAL & PHARMACEUTICAL BULLETIN
Application: ICC
Impact Factor: 1.61
Species: Mouse
PMID: 18981570
Protective effect of bicyclol on acute hepatic failure induced by lipopolysaccharide and d-galactosamine in mice
Published: 2006-02-20
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC
Impact Factor: 2.48
Species: Mouse
PMID: 16487963
Aspirin protected against endothelial damage induced by LDL: role of endogenous NO synthase inhibitors in rats.
Published: 2004-12-01
Journal: ACTA PHARMACOLOGICA SINICA
Impact Factor: 0.88
PMID: 15569408
Effect of systemic LPS injection on cortical NF-κB activity and inflammatory response following traumatic brain injury in rats
Published: 2004-09-16
Journal: BRAIN RESEARCH
Application: IHC
Impact Factor: 2.47
Species: Rat
PMID: 15476694

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Product Promise

We promise Anti-ICAM1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more

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A00171
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 14, 2026