SKU PA1342
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-IL18 Antibody
SKU/Catalog Number PA1342
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Interleukin-18(IL18) detection. Tested with WB in Human.
Cite This Product Anti-IL18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1342)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of Human IL18(175-193aa KKEDELGDRSIMFTVQNED).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

Anti-IL-18 antibody, PA1342, Western blotting
Lane 1: Recombinant Human IL-18 Protein 10ng
Lane 2: Recombinant Human IL-18 Protein 5ng
Lane 3: Recombinant Human IL-18 Protein 2.5ng
Lane 4: RAJI Cell Lysate

Western blot analysis of IL-18 expression in HELA whole cell lysates (lane 1). IL-18 at 22KD was detected using rabbit anti- IL-18 Antigen Affinity purified polyclonal antibody (Catalog # PA1342) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q14116
Gene Name IL18
Protein Name Interleukin-18
Alternative Names Interleukin-18;IL-18;Iboctadekin;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;IL18;IGIF, IL1F4;
Subcellular Localization Secreted.
Molecular Weight 22326 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Research Areas Human

*You can search these to find other products in these research areas.
Background Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide(LPS). After stimulation with IL-18, natural killer(NK) cells and certain T cells release another important cytokine called interferon-gamma(IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein(IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Polyclonal antibody for IL 1F4/IL18 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. IL 1F4/IL18 information: Molecular Weight: 22326 MW; Subcellular Localization: Secreted.
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In stock
Order Product
PA1342
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Publications

The effect of salusin-? on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)
Yang Yj, Shen Y, Chen Sh, Ge Xr. World J Gastroenterol. 2005 Aug 7;11(29):4524-9. Role Of Interleukin 18 In Acute Lung Inflammation Induced By Gut Ischemia Reperfusion.
Wang Y, Zhang X, Zhang Y, Xu H, Fang G. J Reprod Immunol. 2011 Dec;92(1-2):45-53. Doi: 10.1016/J.Jri.2011.07.002. Epub 2011 Sep 13. Expression And Localization Of Il-18 In The Hypothalamic-Pituitary-Ovarian Axis Of Non-Pregnant, Pregnant, And Abor...
Si Lf, Zhang Sy, Gao Cs, Chen Sl, Zhao J, Cheng Xc. Asian-Australas J Anim Sci. 2013 Oct;26(10):1399-405. Doi: 10.5713/Ajas.2013.13101. Effects Of Ifn-?? On Il-18 Expression In Pregnant Rats And Pregnancy Outcomes.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to il 18 antibody, il18 antibody, il-18 antibody
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