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Product Info Summary
| SKU: | M01943 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01943
BM5151 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody catalog # M01943. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01943)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABFI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Calpain 1
Reactive Species
M01943 is reactive to CAPN1 in Human, Mouse, Rat
Observed Molecular Weight
75, 80 kDa
Calculated molecular weight
81.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01943 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Calpain 1 expression in Human fetal kidney lysate.
Specific Publications For Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody (M01943)
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Customer Q&As
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4 Customer Q&As for Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody
Question
We have been able to see staining in human esophagus mucosa. Are there any suggestions? Is anti-Calpain 1 Rabbit Monoclonal antibody supposed to stain esophagus mucosa positively?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Based on literature esophagus mucosa does express CAPN1. Based on Uniprot.org, CAPN1 is expressed in esophagus mucosa, kidney, pancreas placenta, erythroleukemia, liver, among other tissues. Regarding which tissues have CAPN1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pancreas, and Placenta, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-02-20
Question
My colleagues were satisfied with the WB result of your anti-Calpain 1 Rabbit Monoclonal antibody. However we have seen positive staining in esophagus mucosa cytoplasm using this antibody. Is that expected? Could you tell me where is CAPN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
From literature, esophagus mucosa does express CAPN1. Generally CAPN1 expresses in cytoplasm. Regarding which tissues have CAPN1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pancreas, and Placenta, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-04-10
Question
We have tried in the past anti-Calpain 1 Rabbit Monoclonal antibody for WB on kidney a few months ago. I am using mouse, and I plan to use the antibody for IF next. I was wanting to use examining kidney as well as pancreas placenta in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
I viewed the website and datasheets of our anti-Calpain 1 Rabbit Monoclonal antibody and it appears that M01943 has been tested on mouse in both WB and IF. Thus M01943 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-07-31
Question
We are currently using anti-Calpain 1 Rabbit Monoclonal antibody M01943 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
L. Carter
Verified customer
Asked: 2017-05-08
Answer
The anti-Calpain 1 Rabbit Monoclonal antibody (M01943) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-08
