Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody

Boster Bio Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody catalog # M01943. Tested in IF, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M01943
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody

See all Calpain 1 products

SKU/Catalog Number

M01943

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody catalog # M01943. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01943)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABFI-3

Immunogen

A synthesized peptide derived from human Calpain 1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01943 is reactive to CAPN1 in Human, Mouse, Rat

Applications

M01943 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Flow Cytometry 1:100

Validation Images & Assay Conditions

Gene/Protein Information For CAPN1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P07384

Gene ID

823

Gene Name

CAPN1

Full Name

Calpain-1 catalytic subunit

Weight

81.89kDa

Superfamily

peptidase C2 family

Alternative Names

Calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; Calpain mu-type; calpain, large polypeptide L1; calpain-1 catalytic subunit; Calpain-1 large subunit; CANP 1; CANP; CANPL1EC 3.4.22.52; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; EC 3.4.22; micromolar-calpain; muCANPCANP1; muCL CAPN1 CANP, CANP1, CANPL1, SPG76, muCANP, muCL calpain 1 calpain-1 catalytic subunit|CANP 1|calcium-activated neutral proteinase 1|calpain 1, (mu/I) large subunit|calpain mu-type|calpain, large polypeptide L1|calpain-1 large subunit|cell proliferation-inducing gene 30 protein|cell proliferation-inducing protein 30|micromolar-calpain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CAPN1, check out the CAPN1 Infographic

CAPN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CAPN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01943 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen Hx, Tang Sp, Gao Ft, Xu Jl, Jiang Xp, Cao J, Fu Gb, Sun K, Liu Sz, Shi W. Medicine (Baltimore). 2014 Nov;93(23):E138. Doi: 10.1097/Md.0000000000000138. Fibrosis, Adipogenesis, And Muscle Atrophy In Congenital Muscular Torticollis.

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4 Customer Q&As for Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody

Question

We have been able to see staining in human esophagus mucosa. Are there any suggestions? Is anti-Calpain 1 Rabbit Monoclonal antibody supposed to stain esophagus mucosa positively?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

Based on literature esophagus mucosa does express CAPN1. Based on Uniprot.org, CAPN1 is expressed in esophagus mucosa, kidney, pancreas placenta, erythroleukemia, liver, among other tissues. Regarding which tissues have CAPN1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pancreas, and Placenta, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2020-02-20

Question

My colleagues were satisfied with the WB result of your anti-Calpain 1 Rabbit Monoclonal antibody. However we have seen positive staining in esophagus mucosa cytoplasm using this antibody. Is that expected? Could you tell me where is CAPN1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-04-10

Answer

From literature, esophagus mucosa does express CAPN1. Generally CAPN1 expresses in cytoplasm. Regarding which tissues have CAPN1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pancreas, and Placenta, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-04-10

Question

We have tried in the past anti-Calpain 1 Rabbit Monoclonal antibody for WB on kidney a few months ago. I am using mouse, and I plan to use the antibody for IF next. I was wanting to use examining kidney as well as pancreas placenta in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2017-07-31

Answer

I viewed the website and datasheets of our anti-Calpain 1 Rabbit Monoclonal antibody and it appears that M01943 has been tested on mouse in both WB and IF. Thus M01943 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-07-31

Question

We are currently using anti-Calpain 1 Rabbit Monoclonal antibody M01943 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

L. Carter

Verified customer

Asked: 2017-05-08

Answer

The anti-Calpain 1 Rabbit Monoclonal antibody (M01943) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-08

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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M01943
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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