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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||Flow Cytometry, IF, IHC, ICC, WB|
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Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody
Boster Bio Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody catalog # M01943. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calpain 1 CAPN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01943)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human Calpain 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01943 is reactive to CAPN1 in Human, Mouse, Rat
M01943 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Flow Cytometry 1:100
Validation Images & Assay Conditions
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Western blot analysis of Calpain 1 expression in Human fetal kidney lysate (M01943).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPN1 monoclonal antibody (Catalog # M01943) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAPN1
Gene/Protein Information For CAPN1 (Source: Uniprot.Org, NCBI)
Calpain-1 catalytic subunit
peptidase C2 family
Calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; Calpain mu-type; calpain, large polypeptide L1; calpain-1 catalytic subunit; Calpain-1 large subunit; CANP 1; CANP; CANPL1EC 22.214.171.124; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; EC 3.4.22; micromolar-calpain; muCANPCANP1; muCL CAPN1 CANP, CANP1, CANPL1, SPG76, muCANP, muCL calpain 1 calpain-1 catalytic subunit|CANP 1|calcium-activated neutral proteinase 1|calpain 1, (mu/I) large subunit|calpain mu-type|calpain, large polypeptide L1|calpain-1 large subunit|cell proliferation-inducing gene 30 protein|cell proliferation-inducing protein 30|micromolar-calpain*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CAPN1, check out the CAPN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CAPN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M01943 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen Hx, Tang Sp, Gao Ft, Xu Jl, Jiang Xp, Cao J, Fu Gb, Sun K, Liu Sz, Shi W. Medicine (Baltimore). 2014 Nov;93(23):E138. Doi: 10.1097/Md.0000000000000138. Fibrosis, Adipogenesis, And Muscle Atrophy In Congenital Muscular Torticollis.
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