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ICAM1 antibody (M00171-3) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of Caco-2 cells using anti-ICAM1 antibody (M00171-3).
Overlay histogram showing Caco-2 cells stained with M00171-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-ICAM1 Antibody (M00171-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Caco-2 cells stained with M00171-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-ICAM1 Antibody (M00171-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3)
ICAM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IHC, WB |
Tested Applications
ICAM1 antibody was positively detected by WB in:
human Raji whole cell, human Hela whole cell, human K562 whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ICAM1 antibody was positively detected by IHC in:
human ovarian serous adenocarcinoma tissue, human renal carcinoma tissue, human rectal moderately differentiated adenocarcinoma tissue, human spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ICAM1 antibody was positively detected by FC in:
Caco-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3)
View all ICAM1 Antibodies
View all ICAM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6F2C3
Isotype
Mouse IgG1
Immunogen
E. coli-derived human ICAM1 recombinant protein (Position: Q28-R268).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
90-110 kDa
Calculated Molecular Weight
57.8 kDa
Background of ICAM1
CD54, also known as ICAM-1. Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. ICAM-1 is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. This protein is the major human rhinovirus receptor. The ICAM1 gene is mapped to human chromosome 19. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).
Cite This Product
Anti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00171-3)
Protocols
Western Blot protocol for ICAM1 antibody
Immunohistochemistry (IHC) protocol for ICAM1 antibody
Flow Cytometry (FC) protocol for ICAM1 antibody
Publications
Application:
Species:
Malvidin and its Glycosides from Vaccinium ashei Improve Endothelial Function by Anti-inflammatory and Angiotensin I-Converting Enzyme Inhibitory Effects
Published: 2018-01-01
Journal: Natural Product Communications
Application: WB
Impact Factor: 0.81
Species: Human
The Anti-Lung Cancer Activities of Steroidal Saponins of P. polyphylla Smith var. chinensis (Franch.) Hara through Enhanced Immunostimulation in Experimental Lewis Tumor-Bearing C57BL/6 Mice and Induction of Apoptosis in the A549 Cell Line
Published: 2013-10-17
Journal: MOLECULES
Application: WB
Impact Factor: 2.43
Species: Human
PMID: 24141243
Aspirin protected against endothelial damage induced by LDL: role of endogenous NO synthase inhibitors in rats.
Published: 2004-12-01
Journal: ACTA PHARMACOLOGICA SINICA
Impact Factor: 0.88
PMID: 15569408
Review
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Product Promise
We promise Anti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3) performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00171-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026