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Product Info Summary
| SKU: | M09544 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-IDH3A Mouse Monoclonal Antibody [Clone ID: OTI2F11]
SKU/Catalog Number
M09544
Size
100 µl
Description
Boster Bio Anti-IDH3A mouse monoclonal antibody, clone OTI2F11 (formerly 2F11). Catalog# M09544. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-IDH3A Mouse Monoclonal Antibody [Clone ID: OTI2F11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09544)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2F11
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
Reactive Species
M09544 is reactive to IDH3A in Dog, Human, Mouse, Rat
Calculated molecular weight
39.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09544 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH3A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH3A.
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Figure from citation: Western blot analysis of IDH3A protein level by using anti-IDH3A antibody in fibroblasts with miR-424 knockdown or overexpression
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH3A monoclonal antibody.
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Flow cytometric analysis of Hela cells
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Flow cytometric analysis of Jurkat cells
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Anti-IDH3A mouse monoclonal antibody (M09544) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A.
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Immunofluorescent staining of HepG2 cells using anti-IDH3A mouse monoclonal antibody (M09544).
Specific Publications For Anti-IDH3A Mouse Monoclonal Antibody [Clone ID: OTI2F11] (M09544)
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