Product Info Summary
| SKU: | PB9880 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-EPCR/CD201/PROCR Antibody Picoband®
SKU/Catalog Number
PB9880
PB0906 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPCR/CD201/PROCR Antibody Picoband® catalog # PB9880. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPCR/CD201/PROCR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9880)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human EPCR/CD201 recombinant protein (Position: S18-S210). Human EPCR/CD201 shares 65.5% and 65.6% amino acid (aa) sequence identity with mouse and rat EPCR/CD201, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9880 is reactive to PROCR in Human
Observed Molecular Weight
46 kDa, 52 kDa
Calculated molecular weight
26.7 kDa
Background of PROCR
Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor), is a protein that in humans is encoded by the PROCR gene. It has also recently been designated CD201 (cluster of differentiation 201). The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. And it is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9880 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A549 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of EPCR/CD201 using anti-EPCR/CD201 antibody (PB9880).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPCR/CD201 antigen affinity purified polyclonal antibody (Catalog # PB9880) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPCR/CD201 at approximately 46 kDa, 52 kDa. The expected band size for EPCR/CD201 is at 27 kDa.
Click image to see more details
Sandwich ELISA - Recombinant human EPCR protein standard curve.
Use in combination with reagents from Human EPCR ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0957).
Specific Publications For Anti-EPCR/CD201/PROCR Antibody Picoband® (PB9880)
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Customer Q&As
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3 Customer Q&As for Anti-EPCR/CD201/PROCR Antibody Picoband®
Question
Is there a BSA free version of anti-EPCR/CD201/PROCR antibody PB9880 available?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EPCR/CD201/PROCR antibody PB9880 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-30
Question
Is a blocking peptide available for product anti-EPCR/CD201/PROCR antibody (PB9880)?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We do provide the blocking peptide for product anti-EPCR/CD201/PROCR antibody (PB9880). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-29
Question
We are currently using anti-EPCR/CD201/PROCR antibody PB9880 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
The anti-EPCR/CD201/PROCR antibody (PB9880) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-01


