Human EPCR ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Human EPCR. 96wells/kit, with removable strips.
Human EPCR ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human EPCR ELISA Kit PicoKine™
See all EPCR primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Cd201/PROCR with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Cd201/PROCR tissue specificity: Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.|
|Cite This Product||Human EPCR ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0957)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: S18-S210|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For PROCR (Source: Uniprot.org, NCBI)
|NCBI Gene Id||10544|
|Species Of This Entry||Human|
|Protein Name||Endothelial protein C receptor|
|Alternative Names||EPCR|APC receptor; CCCA; CCD41; CCD41centrosome-associated protein; CD201 antigen; CD201; centrocyclin; Endothelial cell protein C receptor; endothelial protein C receptor; EPCR; EPCRMGC23024; PROCR; protein C receptor, endothelial|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Carboxylation; Methylation|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Gene Location||20q11.22, on Chromasome 20, gene sequence: NC_000020.11 (35172072..35215989)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For PROCR (Source: Uniprot.org, NCBI)
|Protein Function||Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.|
|Tissue Specificity||Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.|
|Related Diseases||Inflammation; Thrombosis; Systemic Infection; Malignant Neoplasms; Severe Sepsis; Thromboembolism; Venous Thrombosis; Tissue Adhesions; Neoplasms; Venous Thromboembolism; Inflammatory Response; Thrombophilia; Lupus Erythematosus, Systemic; Blood Coagulation Disorders; Hemorrhage; Infarction; Thrombus; Atherosclerosis; Malignant Neoplasm Of Breast; Mammary Neoplasms|
|Related Pathways||Coagulation; Blood Coagulation; Inflammatory Response; Cell Proliferation; Cell Migration; Chemotaxis; Wound Healing; Hemostasis; Angiogenesis; Cell Adhesion; Pathogenesis; Fibrinolysis; Secretion; Cell Activation; Proteolysis; Immune Response; Cell Cycle; Localization; Cell Death; Chemokine Production|
|Background||Endothelial protein C receptor(EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.|
|Scientific References||PMID: 7929370 by Fukudome K., et al. Identification, cloning, and regulation of a novel endothelial cell protein C/activated protein C receptor.|
PMID: 10397730 by Simmonds R.E., et al. Structural and functional implications of the intron/exon organization of the human endothelial cell protein C/activated protein C receptor (EPCR) gene: comparison with the structure of CD1/major histocompatibility complex alpha1 and alpha2 domains.
Kit Components And Assay QC Data Details Of Human EPCR ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0957-CAP||96-well plate precoated with anti-Human EPCR antibody||1|
|EK0957-ST||lyophilized recombinant Human EPCR standard||20ng/tube|
|EK0957-DA||biotinylated anti-Human EPCR antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0957 Human EPCR ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human EPCR ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human EPCR ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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