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Product Info Summary
| SKU: | EK0957 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human EPCR ELISA Kit PicoKine®
SKU/Catalog Number
EK0957
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human EPCR ELISA Kit PicoKine™ (96 Tests). Quantitate Human PROCR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EPCR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0957)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S18-S210
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0957 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0957 is reactive to PROCR in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0957 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of EPCR
Endothelial protein C receptor (EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0957-CAP | Anti-Human PROCR Pre-coated 96-well strip microplate | 1 |
| EK0957-ST | Human PROCR Standard | 2 vials, 20 ng/tube |
| EK0957-DA | Human PROCR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.028 | 0.142 | 0.272 | 0.462 | 0.733 | 1.185 | 1.722 | 2.062 |
Data Example Images
Click image to see more details
Human EPCR PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect EPCR, Dilution ratio of 1:50, concentration in serum and plasma is around 160 ng/ml..
Some articles we found to cite concentrations of EPCR in samples: 23717683, 21362311 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EPCR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 678 | 1520 | 9734 | 622 | 1487 | 10432 |
| Standard deviation | 44.07 | 91.2 | 486.7 | 49.13 | 96.56 | 678.08 |
| CV (%) | 6.5% | 6% | 5% | 7.9% | 6.4% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of EPCR in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 678 | 818 | 815 | 809 | 780 | 58.97 | 7.5% |
| Sample 2 | 1520 | 1504 | 1453 | 1532 | 1502 | 30.11 | 2% |
| Sample 3 | 9734 | 8679 | 10303 | 9352 | 9517 | 590.41 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For PROCR (Source: Uniprot.Org, NCBI)
Gene Name
PROCR
Full Name
Endothelial protein C receptor
Weight
26.671kDa
Alternative Names
APC receptor; CCCA; CCD41; CCD41centrosome-associated protein; CD201 antigen; CD201; centrocyclin; Endothelial cell protein C receptor; endothelial protein C receptor; EPCR; EPCRMGC23024; PROCR; protein C receptor, endothelial PROCR CCCA, CCD41, EPCR protein C receptor endothelial protein C receptor|APC receptor|CD201 antigen|activated protein C receptor|cell cycle, centrosome-associated protein|centrocyclin|protein C receptor, endothelial
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PROCR, check out the PROCR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PROCR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human EPCR ELISA Kit PicoKine® (EK0957)
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Customer Q&As
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5 Customer Q&As for Human EPCR ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for EPCR ELISA kit? I performed your EPCR ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain EPCR even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-05-26
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this EPCR ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of EPCR that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-05-26
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
J. Chen
Verified customer
Asked: 2018-11-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-05
Question
Q: if the enzyme conjugated EPCR antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the EPCR antigen?
Verified Customer
Verified customer
Asked: 2018-10-18
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-10-18
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
B. Miller
Verified customer
Asked: 2018-08-10
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-08-10
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of EPCR in Human cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2016-08-22
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the EPCR ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-08-22
