|Product Name||Anti-IFITM1 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Interferon-induced transmembrane protein 1(IFITM1) detection. Tested with WB, IHC-P, IHC-F in Human.|
|Cite This Product||Anti-IFITM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1112)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human IFITM1(1-17aa MHKEEHEVAVLGPPPST).|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Anti-IFITM1 antibody, PA1112, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: CEM Cell Lysate
Anti-IFITM1 antibody, PA1112, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Interferon-induced transmembrane protein 1|
|Tissue Specificity||Bone (at protein level). Levels greatly elevated in colon cancer, cervical cancer, esophageal cancer and ovarian cancer. Expressed in glioma cell lines. .|
|Alternative Names||Interferon-induced transmembrane protein 1;Dispanin subfamily A member 2a;DSPA2a;Interferon-induced protein 17;Interferon-inducible protein 9-27;Leu-13 antigen;CD225;IFITM1;CD225, IFI17;|
|Subcellular Localization||Cell membrane ; Single-pass membrane protein .|
|Molecular Weight||13964 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV). Can inhibit: influenza virus hemagglutinin protein- mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation. .|
|Research Areas||Adapters, Cancer, Cytokines, Immunology, Innate Immunity, Interferons, Oncoproteins/Suppressors, P53 Pathway, Signal Transduction, Signaling Pathways, Stem Cells, Surface Molecules, Tgf Beta, Transmembrane, Tumor Biomarkers, Tumor Suppressors
*You can search these to find other products in these research areas.
|Background||Interferon-induced Transmembrane Protein 1(IFITM1), also called Interferon-induced Protein 17(IFI17). IFITM1 activity is required for primordial germ cells(PGCs) transit from the mesoderm into the endoderm, and that it appears to act via a repulsive mechanism, such that PGCs avoid Ifitm1-expressing tissues. It is mapped to Chr.11 and belongs to the family of interferon-induced transmembrane proteins(Ifitm/mil/fragilis), which encodes cell surface proteins that may modulate cell adhesion and influence cell differentiation. Interferon-inducible membrane proteins of approximately 17 kDa have been suggested to play a role in the antiproliferative activity of interferons based on their pattern of induction in interferon-sensitive and -resistant cell lines and the ability of a membrane fraction enriched in 17-kDa proteins to inhibit cell growth.|
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (PA1112)
Promotion:Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,