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Product Info Summary
| SKU: | H01857 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated
SKU/Catalog Number
H01857
BM3897 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated catalog # H01857. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H01857)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DF-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta Tubulin
Reactive Species
H01857 is reactive to TUBB3 in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
50.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
H01857 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
Positive Control
WB: Jurkat cell, Human kidney, 3T3 cell, Mouse brain, C6 cell, Rat heart (H01857)
Validation Images & Assay Conditions
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Western blot analysis of beta Tubulin expression in (1) Jurkat cell lysate, (2) Human kidney lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) C6 cell lysate, (6) Rat heart lysate (H01857).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody (Catalog # H01857) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB3
Specific Publications For Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated (H01857)
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