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Product Info Summary
| SKU: | M00070-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-IGF1 Receptor (IGF1R) Mouse Monoclonal Antibody [Clone ID: OTI4C4]
SKU/Catalog Number
M00070-1
Size
100 µl
Description
Boster Bio IGF1R mouse monoclonal antibody, clone OTI4C4 (formerly 4C4). Catalog# M00070-1. Tested in FC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-IGF1 Receptor (IGF1R) Mouse Monoclonal Antibody [Clone ID: OTI4C4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00070-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4C4
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 741-935 of human IGF1R (NP_000866) produced in Insect.
Reactive Species
M00070-1 is reactive to IGF1R in Human, Mouse, Rat
Calculated molecular weight
154.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00070-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF1R (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF1R (1:2000).
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Western blot analysis of extracts (35ug) from 4 cell lines lysates by using anti-IGF1R monoclonal antibody (1:500).
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Living HEK293T cells transfected with either IGF1R (Myc-DDK-tagged) overexpress plasmid (Red)
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Flow cytometric analysis of living HepG2 cells
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Flow cytometric analysis of living A549 cells
Specific Publications For Anti-IGF1 Receptor (IGF1R) Mouse Monoclonal Antibody [Clone ID: OTI4C4] (M00070-1)
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