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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human IGF1R/Igf1 Receptor ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human IGF1R/Igf1 Receptor ELISA Kit PicoKine™ (96 Tests). Quantitate Human IGF1R in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IGF1R/Igf1 Receptor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0776)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E31-N932
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0776 is reactive to IGF1R in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0776 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGF-I R/IGF1R
IGF1R, also called IGFR or insulin-like growth factor 1 receptor, is a protein that found on the surface of human cells. This gene is mapped to 15q26.3. IGF1R belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. IGF1R also plays a critical role in transformation events. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
|EK0776-CAP||Anti-Human IGF1R Pre-coated 96-well strip microplate||1|
|EK0776-ST||Human IGF1R Standard||2 vials, 10 ng/tube|
|EK0776-DA||Human IGF1R Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human IGF1R PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGF-I R/IGF1R, Dilution ratio of 1:1, concentration in serum is around 500pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IGF1R/Igf1 Receptor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IGF-I R/IGF1R in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For IGF1R (Source: Uniprot.Org, NCBI)
Insulin-like growth factor 1 receptor
protein kinase superfamily
CD221 antigen; CD221; EC 2.7.10; EC 184.108.40.206; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2 IGF1R CD221, IGFIR, IGFR, JTK13 insulin like growth factor 1 receptor insulin-like growth factor 1 receptor|IGF-I receptor|soluble IGF1R variant 1|soluble IGF1R variant 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IGF1R, check out the IGF1R Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGF1R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0776 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Alashti, M.,Shirvani, H.,Sabzevari Rad,R. et al.Effects of different small-sided games on blood lactate and GH/IGF-1 axis responses in young soccer players.Ger J Exerc Sport Res(2020).https://doi.org/10.1007/s1266 2-020-00691-6
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