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- Table of Contents
Il16 antibody (A01635) performs well in ELISA, IHC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of IL-16 using anti-IL-16 antibody (A01635). IL-16 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL-16 Antibody (A01635) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-IL-16/Il16 Antibody Picoband®
Il16 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, IHC, WB |
Tested Applications
Il16 antibody was positively detected by IHC in:
mouse brain tissue, rat brain tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse,
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Mouse
Recommend
Tested
Recommend
Recommend
Rat
Tested
Recommend
Product Information
Product Name
Anti-IL-16/Il16 Antibody Picoband®
View all IL16 Antibodies
View all IL16 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL-16 recombinant protein (Position: S1205-S1322). Mouse IL-16 shares 86.7% amino acid (aa) sequence identity with human IL-16.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated Molecular Weight
141.4 kDa
Background of Il16
Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. It is mapped to 15q25.1. IL-16 was originally described as a factor that could attract activated T cells in humans. It was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16 stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
Cite This Product
Anti-IL-16/Il16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01635)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
91%
High Homology
Human
88%
Moderate Homology
Monkey
87%
Moderate Homology
Sheep
81%
Moderate Homology
Cow
80%
Moderate Homology
Dog
78%
Moderate Homology
Rabbit
74%
Moderate Homology
Drosophila
37%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for Il16 antibody
Publications
Application:
Species:
Enhanced interleukin-16-CD4 signaling in CD3 T cell mediates neuropathic pain via activating astrocytes in female mice
Published: 2024-08-11
Journal: NEUROPHARMACOLOGY
Application: IF
Impact Factor: 4.60
Species: Mouse
PMID: 39137872
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Product Promise
We promise Anti-IL-16/Il16 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A01635
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026