Anti-IL6 Antibody

Rabbit Polyclonal IL6 Antibody. Validated in WB and tested in Human.

SKU PA1700
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-IL6 Antibody
See all IL6 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1700
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal IL6 Antibody. Validated in WB and tested in Human. Size: 100μg/vial.
Cite This Product Anti-IL6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1700)
Specificity Anti-IL6 Antibody (PA1700) reacts with Human IL6, in native form and recombinant. Superfamily members of IL6 are not reactive to PA1700.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-IL6 Antibody (PA1700) was raised against A synthetic peptide corresponding to a sequence at the C-terminus of human IL-6(195-212aa LRSFKEFLQSSLRALRQM)..
Reactivity Human

Assay Details

Our Boster Quality Guarantee for Anti-IL6 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-IL6 Antibody (PA1700).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1700 1 WB anti il 6 antibody.jpg

Anti-IL-6 antibody, PA1700, Western blotting
Lane 1: Recombinant Human IL-6 Protein 10ng
Lane 2: Recombinant Human IL-6 Protein 5ng
Lane 3: Recombinant Human IL-6 Protein 2.5ng

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P05231
Gene Name IL6
Protein Name Interleukin-6
Alternative Names Interleukin-6;IL-6;B-cell stimulatory factor 2;BSF-2;CTL differentiation factor;CDF;Hybridoma growth factor;Interferon beta-2;IFN-beta-2;IL6;IFNB2;Show more
Subcellular Localization Secreted.
Molecular Weight 23718 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.
Research Areas Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology

*You can search these to find other products in these research areas.
Background Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al.(1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al.(1988) mapped the IL6 gene to chromosome 7p15.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-IL6 Antibody (PA1700)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1700)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships in 7-10 business days.

Troubleshooting

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We promise all of our products perform as described in datasheets.

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Publications

Efficacy of acetylshikonin in preventing obesity and hepatic steatosis in db/db mice
Rhein inhibits lipopolysaccharide-induced intestinal injury during sepsis by blocking the toll-like receptor 4 nuclear factor-%u03BAB pathway
Effects of triptolide from Radix Tripterygium wilfordii (Leigongteng) on cartilage cytokines and transcription factor NF-%u03BAB: a study on induced arthritis in rats
Distribution change of mast cells in human nasal polyps
Involvement of tumor necrosis factor-%u03B1 in the upregulation of CXCR4 expression in gastric cancer induced by Helicobacter pylori
Serum interleukin-6 is not linked with sleep-quality, restless legs syndrome, and depression, but with 6-month survival in hematological malignancies
Expression of the interleukin 6 system in cortical lesions from patients with tuberous sclerosis complex and focal cortical dysplasia type IIb
Expression of the interleukin 6 system in cortical lesions from patients with tuberous sclerosis complex and focal cortical dysplasia type IIb
Expression of the interleukin 6 system in cortical lesions from patients with tuberous sclerosis complex and focal cortical dysplasia type IIb
The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway
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Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication
The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells
Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-?B pathway
Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells
Angiotensin-(1-7) inhibits inflammation and oxidative stress to relieve lung injury induced by chronic intermittent hypoxia in rats
Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity
MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3
Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway
Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression
Lu Y, Zhang XS, Zhang ZH, Zhou XM, Gao YY, Liu GJ, Wang H, Wu LY, Li W, Hang CH. J Neuroinflammation. 2018 Mar 19;15(1):87. doi: 10.1186/s12974-018-1118-4. Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarach...
Liao Q, Zeng Z, Guo X, Li X, Wei F, Zhang W, Li X, Chen P, Liang F, Xiang B, Ma J, Wu M, Tang H, Deng M, Zeng X, Tang K, Xiong W, Li G. Oncogene. 2014 Apr 17;33(16):2098-109. Doi: 10.1038/Onc.2013.161. Epub 2013 May 27. Lplunc1 Suppresses Il-6-Ind...
Cui L, Feng L, Zhang Zh, Jia Xb. Int Immunopharmacol. 2014 Nov;23(1):294-303. Doi: 10.1016/J.Intimp.2014.09.005. Epub 2014 Sep 18. The Anti-Inflammation Effect Of Baicalin On Experimental Colitis Through Inhibiting Tlr4/Nf-??b Pathway Activation.
Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.
Ye Q, Zheng Y, Fan S, Qin Z, Li N, Tang A, Ai F, Zhang X, Bian Y, Dang W, Huang J, Zhou M, Zhou Y, Xiong W, Yan Q, Ma J, Li G. Plos One. 2014 Jul 24;9(7):E103298. Doi: 10.1371/Journal.Pone.0103298. Ecollection 2014. Lactoferrin Deficiency Promotes...
Zhao H, Li X, Li N, Liu T, Liu J, Li Z, Xiao H, Li J. Br J Nutr. 2014 Mar 14;111(5):836-46. Doi: 10.1017/S0007114513003115. Epub 2013 Sep 30. Long-Term Resveratrol Treatment Prevents Ovariectomy-Induced Osteopenia In Rats Without Hyperplastic Effe...
Li J, Zhang J, Fu Y, Sun X, Gong T, Jiang J, Zhang Z. J Control Release. 2015 Aug 28;212:19-29. Doi: 10.1016/J.Jconrel.2015.06.011. Epub 2015 Jun 11. Dual Pancreas- And Lung-Targeting Therapy For Local And Systemic Complications Of Acute Pancreati...
Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.
Huang Q, Du J, Fan J, Lv Z, Qian X, Zhang X, Han J, Chen C, Wu F, Jin Y. Med Oncol. 2014 Sep;31(9):144. Doi: 10.1007/S12032-014-0144-Z. Epub 2014 Aug 12. The Effect Of Proinflammatory Cytokines On Il-17Ra Expression In Nsclc.
Liu T, Zeng Z, Liu Y, Wang J, Maitz Mf, Wang Y, Liu S, Chen J, Huang N. Acs Appl Mater Interfaces. 2014 Jun 11;6(11):8729-43. Doi: 10.1021/Am5015309. Epub 2014 Apr 23. Surface Modification With Dopamine And Heparin/Poly-L-Lysine Nanoparticles Prov...
Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...
Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.
Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Bear...
Zhu J, Mu X, Zeng J, Xu C, Liu J, Zhang M, Li C, Chen J, Li T, Wang Y. Plos One. 2014 Jun 30;9(6):E101291. Doi: 10.1371/Journal.Pone.0101291. Ecollection 2014. Ginsenoside Rg1 Prevents Cognitive Impairment And Hippocampus Senescence In A Rat Model...
Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.
Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.
Sun W, Liu Db, Li Ww, Zhang Ll, Long Gx, Wang Jf, Mei Q, Hu Gq. Int J Oncol. 2014 May;44(5):1551-60. Doi: 10.3892/Ijo.2014.2323. Epub 2014 Mar 5. Interleukin-6 Promotes The Migration And Invasion Of Nasopharyngeal Carcinoma Cell Lines And Upregula...
Hu W,, Jing P, Wang L, Zhang Y, Yong J, Wang Y. Bmc Complement Altern Med. 2015 Apr 15;15:119. Doi: 10.1186/S12906-015-0642-3. The Positive Effects Of Ginsenoside Rg1 Upon The Hematopoietic Microenvironment In A D-Galactose-Induced Aged Rat Model.
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...
Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to il 6 antibody, il-6 antibody, il6 antibody, interleukin 6 antibody, interleukin-6 antibody