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Product Info Summary
| SKU: | PA1700 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interleukin-6 IL6 Antibody Picoband®
SKU/Catalog Number
PA1700
BA0562-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-6 IL6 Antibody catalog # PA1700. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-6 IL6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1700)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IL-6.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1700 is reactive to IL6 in Human
Observed Molecular Weight
24 kDa
Calculated molecular weight
23.7 kDa
Background of IL6
Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al. (1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) mapped the IL6 gene to chromosome 7p15.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1700 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: recombinant human IL6 protein
Validation Images & Assay Conditions
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Western blot analysis of IL6 using anti-IL6 antibody (PA1700).
Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours.
Lane 1: recombinant human IL6 protein 5 ng,
Lane 2: recombinant human IL6 protein 2.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1700) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL6 at approximately 24 kDa.
Specific Publications For Anti-Interleukin-6 IL6 Antibody Picoband® (PA1700)
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Customer Q&As
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3 Customer Q&As for Anti-Interleukin-6 IL6 Antibody Picoband®
Question
We have been able to see staining in human lung. Do you have any suggestions? Is anti-IL6 antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
Based on literature lung does express IL6. Based on Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-30
Question
We are currently using anti-IL6 antibody PA1700 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
D. Edwards
Verified customer
Asked: 2017-08-15
Answer
The anti-IL6 antibody (PA1700) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-15
Question
We were happy with the WB result of your anti-IL6 antibody. However we have seen positive staining in fibroblast secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?
T. Jones
Verified customer
Asked: 2016-12-28
Answer
From what I have seen in literature, fibroblast does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-12-28
