Human IL-6 PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human IL-6 PicoKine® Quick ELISA Kit

See all IL6 products

SKU/Catalog Number

FEK0410

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-6 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human IL6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human IL-6 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0410)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: P29-M212

Capture/Detection Antibodies

The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.

Sensitivity

<0.3 pg/ml

Assay Range

4.69 pg/ml - 300 pg/ml

Standard Dilution Instructions

See datasheet of FEK0410 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0410 is reactive to IL6 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

FEK0410 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL6

The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0410-CAP	Anti-Human IL6 Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0410-ST	Human IL6 Standard	2	10 ng/tube
FEK0410-H	HRP Linked Anti-Human IL6 Antibody	1	6ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	4.69	9.38	18.75	37.5	75	150	300
O.D.	0.006	0.081	0.145	0.276	0.486	0.952	1.513	2.074

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-6 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Gene/Protein Information For IL6 (Source: Uniprot.Org, NCBI)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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