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Product Info Summary
| SKU: | M00651 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IL1RA Rabbit Monoclonal Antibody
SKU/Catalog Number
M00651
BM4628 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IL1RA Rabbit Monoclonal Antibody catalog # M00651. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL1RA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00651)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GEI-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IL1RA
Reactive Species
M00651 is reactive to IL1RN in Human, Mouse, Rat
Observed Molecular Weight
20 kDa
Calculated molecular weight
20.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00651 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: HeLa cell, 3T3 cell
Validation Images & Assay Conditions
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Western blot analysis of IL1RA expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
Specific Publications For Anti-IL1RA Rabbit Monoclonal Antibody (M00651)
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3 Customer Q&As for Anti-IL1RA Rabbit Monoclonal Antibody
Question
Is this M00651 anti-IL1RA Rabbit Monoclonal antibody reactive to the isotypes of IL1RN?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The immunogen of M00651 anti-IL1RA Rabbit Monoclonal antibody is A synthesized peptide derived from human IL1RA . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-10
Question
I was wanting to use your anti-IL1RA Rabbit Monoclonal antibody for WB for human lower esophagus mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lower esophagus mucosa identification?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
You can see on the product datasheet, M00651 anti-IL1RA Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lower esophagus mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-27
Question
Will anti-IL1RA Rabbit Monoclonal antibody M00651 work on pig IP with pancreas?
B. Taylor
Verified customer
Asked: 2017-10-16
Answer
Our lab technicians have not validated anti-IL1RA Rabbit Monoclonal antibody M00651 on pig. You can run a BLAST between pig and the immunogen sequence of anti-IL1RA Rabbit Monoclonal antibody M00651 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig pancreas in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-16
