Rat CRP/C Reactive Protein ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CRP. 96wells/kit, with removable strips. Cited in 13 publication(s).
Rat CRP/C Reactive Protein ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Rat CRP/C Reactive Protein ELISA Kit PicoKine™
See all C-Reactive Protein/CRP primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Rat C-Reactive Protein/CRP with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. C-Reactive Protein/CRP tissue specificity: Found in plasma.|
|Cite This Product||Rat CRP/C Reactive Protein ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0978)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: H20-S230|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Crp (Source: Uniprot.org, NCBI)
|NCBI Gene Id||25419|
|Species Of This Entry||Rat|
|Protein Name||C-reactive protein|
|Alternative Names||C-Reactive Protein/CRP|C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895|
|Post Tranlational Modifications||Phosphorylation; Glycosylation; Oxidation; Cleavage; Citrullination; Nitration; Reduction; Biotinylation; Methylation; Acylation; Acetylation; Carboxylation; Dephosphorylation; Iodination; Amination; Deamination; Ubiquitination; Glutathionylation; Deacetylation; Geranylgeranylation|
|Gene Location||13q24, on Chromasome 13, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Crp (Source: Uniprot.org, NCBI)
|Protein Function||Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity). .|
|Tissue Specificity||Found in plasma.|
|Related Diseases||Inflammation; Cardiovascular Diseases; Arthritis; Diabetes Mellitus; Atherosclerosis; Rheumatoid Arthritis; Obesity; Infective Disorder; Inflammatory Response; Malignant Neoplasms; Infarction; Myocardial Infarction; Hypertensive Disease; Pain; Coronary Artery Disease; Heart Diseases; Coronary Heart Disease; Insulin Resistance; Systemic Infection; Pneumonia|
|Related Pathways||Inflammatory Response; Pathogenesis; Secretion; Coagulation; Immune Response; Cell Adhesion; Excretion; Acute-phase Response; Glomerular Filtration; Complement Activation; Aging; Transport; Virulence; Platelet Activation; Localization; Platelet Aggregation; Fibrinolysis; Cytokine Production; Angiogenesis; Phagocytosis|
|Background||C Reactive Protein(CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.|
|Scientific References||PMID: 1737750 by Rassouli M., et al. Derivation of the amino acid sequence of rat C-reactive protein from cDNA cloning with additional studies on the nature of its dimeric component.|
Kit Components And Assay QC Data Details Of Rat CRP/C Reactive Protein ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0978-CAP||96-well plate precoated with anti-Rat C-Reactive Protein/CRP antibody||1|
|EK0978-ST||lyophilized recombinant Rat C-Reactive Protein/CRP standard||10ng/tube|
|EK0978-DA||biotinylated anti-Rat C-Reactive Protein/CRP antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0978 Rat CRP/C Reactive Protein ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Rat CRP/C Reactive Protein ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Rat CRP/C Reactive Protein ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 13
|Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca...||PubMed ID 22509346|
|Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.||PubMed ID 24137221|
|Asgary S, Keshvari M, Sahebkar A, Hashemi M, Rafieian-Kopaei M. Arya Atheroscler. 2013 Nov;9(6):326-31. Clinical Investigation Of The Acute Effects Of Pomegranate Juice On Blood Pressure And Endothelial Function In Hypertensive Individuals.||PubMed ID 24575134|
|Zou X, Huang W, Lu F, Fang K, Wang D, Zhao S, Jia J, Xu L, Wang K, Wang N, Dong H. BMC Complement Altern Med. 2017 Mar 23;17(1):165. doi: 10.1186/s12906-017-1648-9.The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesit...||PubMed ID 28335761|
|Wang Y, Chai Y, He X, Ai L, Sun X, Huang Y, Li Y. Exp Ther Med. 2017 Oct;14(4):3463-3470. doi: 10.3892/etm.2017.4971. Epub 2017 Aug 18. Intermittent hypoxia simulating obstructive sleep apnea causes pulmonary inflammation and activates the Nrf2/HO...||PubMed ID 29042934|
|Li Y, Su R, Xu S, Huang Q, Xu H. Neuropsychiatr Dis Treat. 2017 Sep 27;13:2477-2487. doi: 10.2147/NDT.S145069. eCollection 2017. Artesunate prevents rats from the clozapine-induced hepatic steatosis and elevation in plasma triglycerides||PubMed ID 29026311|
|Liao N, Zheng Y, Xie H, Zhao B, Zeng Y, Liu X, Liu J. Stem Cell Res Ther. 2017 Dec 19;8(1):286. doi: 10.1186/s13287-017-0743-7. Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabeti...||PubMed ID 29258603|
|Sequential Treatments with Tongsai and Bufei Yishen Granules Reduce Inflammation and Improve Pulmonary Function in Acute Exacerbation-Risk Window of Chronic Obstructive Pulmonary Disease in Rats||PubMed ID 27563333|
|Dendrobium Officinale Kimura et Migo Ameliorates Insulin Resistance in Rats with Diabetic Nephropathy||PubMed ID 29849017|
|The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation||PubMed ID 30128882|
|Potential role of lycopene in targeting proprotein convertase subtilisin/kexin type-9 to combat hypercholesterolemia||PubMed ID 28412198|
|Amelioration of PM2.5-induced lung toxicity in rats by nutritional supplementation with fish oil and Vitamin E||PubMed ID 30992001|
|Dynamic Characteristics of Sequential Acute Exacerbations and Risk Windows in AECOPD Rats Induced by Cigarette-Smoke and Exposure to Klebsiella pneumoniae||PubMed ID 30058599|
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