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- Table of Contents
IL23A antibody (A01097-1) performs well in ELISA, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Western blot analysis of IL23 using anti-IL23 antibody (A01097-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PANC-1 cell lysates,
Lane 3: rat lymph tissue lysates,
Lane 4: rat small intestine tissue lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat ovary tissue lysates,
Lane 7: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PANC-1 cell lysates,
Lane 3: rat lymph tissue lysates,
Lane 4: rat small intestine tissue lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat ovary tissue lysates,
Lane 7: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
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Anti-IL23/IL23A Antibody Picoband®
IL23A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, WB |
Tested Applications
IL23A antibody was positively detected by WB in:
human placenta tissue, human PANC-1 cell, rat lymph tissue, rat small intestine tissue, rat testis tissue, rat ovary tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-IL23/IL23A Antibody Picoband®
View all IL23A Antibodies
View all IL23A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL23 recombinant protein (Position: R20-R178).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated Molecular Weight
20.7 kDa
Background of IL23A
Interleukin-23 subunit alpha is a protein that in humans is encoded by the IL23A gene. IL-23, also known as Interleukin-23 subunit alphainin (IL23A), is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). The International Radiation Hybrid Mapping Consortium mapped the IL-23 gene to chromosome 12. IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration.
Cite This Product
Anti-IL23/IL23A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01097-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
89%
Moderate Homology
Dog
88%
Moderate Homology
Rat
81%
Moderate Homology
Cow
80%
Moderate Homology
Sheep
79%
Moderate Homology
Mouse
75%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for IL23A antibody
Publications
Application:
Species:
Di-(2-ethylhexyl) phthalate aggravates psoriasis-like skin lesions: In vitro and in vivo evaluation
Published: 2023-10-01
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: WB
Impact Factor: 3.80
Species: Human
PMID: 37783235
IL-23/IL-17 immune axis mediates the imiquimod-induced psoriatic inflammation by activating ACT1/TRAF6/TAK1/NF-κB pathway in macrophages and keratinocytes
Published: 2023-04-26
Journal: KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
Impact Factor: 3.30
Species: Mouse
PMID: 37098777
IL-17 Aggravates Pseudomonas aeruginosa Airway Infection in Acute Exacerbations of Chronic Obstructive Pulmonary Disease
Published: 2022-01-13
Journal: Frontiers in Immunology
Application: WB
Impact Factor: 8.79
Species: Mouse
PMID: 35095906
Effects of Bacillus subtilis on Epithelial Tight Junctions of Mice with Inflammatory Bowel Disease
Published: 2016-02-02
Journal: JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
Application: WB
Impact Factor: 2.14
Species: Mouse
PMID: 26720180
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Product Promise
We promise Anti-IL23/IL23A Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01097-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026