Click image to see more details
Product Info Summary
| SKU: | A03158-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband®
SKU/Catalog Number
A03158-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband® catalog # A03158-2. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03158-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse IFN gamma Receptor beta/AF-1/IFNGR2 recombinant protein (Position: A20-P332).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03158-2 is reactive to IFNGR2 in Mouse
Observed Molecular Weight
55 kDa
Background of IFNGR2
Interferon gamma receptor 2 also known as IFN-γR2 is a protein which in humans is encoded by the IFNGR2 gene. It is mapped to 16 C3.3; 16 53.07 cM. This gene (IFNGR2) encodes the non-ligand-binding beta chain of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03158-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: mouse thymus tissue, mouse lung tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IFN Gamma Receptor Beta/AF-1/Ifngr2 using anti-IFN Gamma Receptor Beta/AF-1/Ifngr2 antibody (A03158-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN Gamma Receptor Beta/AF-1/Ifngr2 antigen affinity purified polyclonal antibody (Catalog # A03158-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFN Gamma Receptor Beta/AF-1/Ifngr2 at approximately 55KD. The expected band size for IFN Gamma Receptor Beta/AF-1/Ifngr2 is at 38KD.
Specific Publications For Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband® (A03158-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
