Human IL-18R1 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-18R1. 96wells/kit, with removable strips.
Human IL-18R1 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human IL-18R1 ELISA Kit PicoKine™
See all IL18R1 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Il-1 R5/IL18R1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il-1 R5/IL18R1 tissue specificity: Expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression in Hodgkin disease cell lines.|
|Cite This Product||Human IL-18R1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1260)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: C22-R329|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For IL18R1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||8809|
|Species Of This Entry||Human|
|Protein Name||Interleukin-18 receptor 1|
|Superfamily||interleukin-1 receptor family|
|Alternative Names||IL18R1|CD218a; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Acetylation; Methylation; Glycosylation|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Gene Location||2q12.1, on Chromasome 2, gene sequence: NC_000002.12 (102355796..102398777)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL18R1 (Source: Uniprot.org, NCBI)
|Protein Function||Within the IL18 receptor complex, responsible for the binding of the proinflammatory cytokine IL18, but not IL1A nor IL1B (PubMed:8626725, PubMed:14528293, PubMed:25261253, PubMed:25500532). Involved in IL18-mediated IFNG synthesis from T- helper 1 (Th1) cells (PubMed:10653850). Contributes to IL18- induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3 (By similarity).|
|Tissue Specificity||Highly expressed in leukocytes, spleen, lung. Also expressed, but at lower levels, in liver, small intestine, colon, prostate, thymus, placenta, and heart. Specifically coexpressed with IL18R1 in Th1 cells (PubMed:10925275, PubMed:11046021, PubMed:10653850).|
|Related Diseases||Inflammation; Neoplasms; Rheumatoid Arthritis; Autoimmune Reaction; Arthritis; Asthma; Autoimmune Diseases; Infective Disorder; Malignant Neoplasms; Degenerative Polyarthritis; Innate Immune Response; Autoimmunity; Tissue Adhesions; Crohn Disease; Graft-vs-host Disease; Hepatitis; Inflammatory Bowel Diseases; Carcinoma; Psoriasis; Communicable Diseases|
|Related Pathways||Immune Response; Pathogenesis; Secretion; Cytokine Production; Cell Proliferation; Cell Activation; Interferon-gamma Production; Innate Immune Response; Cell Development; T Cell Activation; Cell Differentiation; Histone Acetylation; Inflammatory Response; Chemokine Production; Cell Maturation; Glycosylation; Hypersensitivity; Adaptive Immune Response; Reverse Transcription; Methylation|
|Background||The interleukin-18 receptor 1(IL-18R1), also known as CDw218a(cluster of differentiation w218a) or IL18RA, is an interleukin receptor of the immunoglobulin superfamily. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2(IL-1Rrp2), and IL1RL1(T1/ST2), form a gene cluster on chromosome 2q. This receptor specifically binds interleukin 18(IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells.|
|Scientific References||PMID: 8626725 by Parnet P., et al. IL-1Rrp is a novel receptor-like molecule similar to the type I interleukin-1 receptor and its homologues T1/ST2 and IL-1R AcP.|
PMID: 9325300 by Torigoe K., et al. Purification and characterization of the human interleukin-18 receptor.
Kit Components And Assay QC Data Details Of Human IL-18R1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1260-CAP||96-well plate precoated with anti-Human IL18R1 antibody||1|
|EK1260-ST||lyophilized recombinant Human IL18R1 standard||10ng/tube|
|EK1260-DA||biotinylated anti-Human IL18R1 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1260 Human IL-18R1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IL-18R1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human IL-18R1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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