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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human IL-22/Interleukin-22 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human IL-22 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-22/Interleukin-22 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0933)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: A34-I179
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0933 is reactive to IL22 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0933 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-22
Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
|EK0933-CAP||Anti-Human IL22 Pre-coated 96-well strip microplate||1|
|EK0933-ST||Human IL22 Standard||2 vials, 10 ng/tube|
|EK0933-DA||Human IL22 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human IL-22 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-22, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Some articles we found to cite concentrations of IL-22 in samples: 25079457 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-22/Interleukin-22 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IL-22 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For IL22 (Source: Uniprot.Org, NCBI)
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18 IL22 IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18 interleukin 22 interleukin-22|IL-10-related T-cell-derived inducible factor|cytokine Zcyto18*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL22, check out the IL22 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0933 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Correlation between intestinal flora and serum inflammatory factors in patients with Crohns disease.
‘Psoriasis 1’ reduces psoriasis‑like skin inflammation by inhibiting the VDR‑mediated nuclear NF‑κB and STAT signaling pathways
Gioxari A,Amerikanou C,Papada E,Zioga E,Georgoulis AD,Bamias G,Kaliora AC.Serum Vitamins D, B9 and B12 in Greek Patients with Inflammatory Bowel Diseases. Nutrients. 2020 Dec 4;12(12):E3734.doi:10.3390 /nu12123734.PMID:33291539.
EK0933 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Psoriasis 1'reduces psoriasis%u2011like skin inflammation by inhibiting the VDR%u2011mediated nuclear NF%u2011%u03BAB and STAT signaling pathways
Min M, Yan BX, Wang P, Landeck L, Chen JQ, Li W, Cai SQ, Zheng M, Man XY. PLoS One. 2017 Dec 22;12(12):e0190051. doi: 10.1371/journal.pone.0190051. eCollection 2017. Rottlerin as a therapeutic approach?in psoriasis: Evidence from in vitro and in v...
Chai H, Yang L, Gao L, Guo Y, Li H, Fan X, Wu B, Xue S, Cai Y, Jiang X, Qin B, Zhang S, Ke Y. Transplantation. 2014 Oct 15;98(7):729-37. Doi: 10.1097/Tp.0000000000000319. Decreased Percentages Of Regulatory T Cells Are Necessary To Activate Th1-Th...
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