Human IL-22 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-22. 96wells/kit, with removable strips. Cited in 3 publication(s).
Human IL-22 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human IL-22 ELISA Kit PicoKine™
See all IL-22 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human IL22 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL22 tissue specificity:|
|Cite This Product||Human IL-22 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0933)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: A34-I179|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For IL22 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||50616|
|Species Of This Entry||Human|
|Alternative Names||IL-22|Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18|
|Post Tranlational Modifications||Phosphorylation; Glycosylation|
|Gene Location||12q15, on Chromasome 12, gene sequence: NC_000012.12 (68248242..68253604, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL22 (Source: Uniprot.org, NCBI)
|Protein Function||Cytokine that contributes to the inflammatory response in vivo.|
|Interacting Proteins||IL10RB; IL22RA1|
|Related Diseases||Inflammation; Autoimmune Reaction; Autoimmune Diseases; Psoriasis; Crohn Disease; Colitis; Autoimmunity; Inflammatory Bowel Diseases; Intestinal Diseases; Ulcer; Chronic Inflammation; Encephalomyelitis; Ulcerative Colitis; Neoplasms; Dysequilibrium Syndrome; Dermatologic Disorders; Experimental Autoimmune Encephalomyelitis; Sclerosis; Polyps|
|Related Pathways||Pathogenesis; Immune Response; Cytokine Production; Cell Differentiation; Secretion; Lactation; Glycosylation; Cytokine Secretion; Pigmentation; Adaptive Immune Response; Localization; Decidualization; Cell Division; Cell Motility; Angiogenesis|
|Background||Interleukin-22(IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.|
|Scientific References||PMID: 10954742 by Dumoutier L., et al. Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte- stimulating factor.|
PMID: 11197690 by Dumoutier L., et al. IL-TIF/IL-22: genomic organization and mapping of the human and mouse genes.
Kit Components And Assay QC Data Details Of Human IL-22 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0933-CAP||96-well plate precoated with anti-Human IL-22 antibody||1|
|EK0933-ST||lyophilized recombinant Human IL-22 standard||10ng/tube|
|EK0933-DA||biotinylated anti-Human IL-22 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0933 Human IL-22 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IL-22 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human IL-22 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 3
|Chai H, Yang L, Gao L, Guo Y, Li H, Fan X, Wu B, Xue S, Cai Y, Jiang X, Qin B, Zhang S, Ke Y. Transplantation. 2014 Oct 15;98(7):729-37. Doi: 10.1097/Tp.0000000000000319. Decreased Percentages Of Regulatory T Cells Are Necessary To Activate Th1-Th...||PubMed ID 25093516|
|Min M, Yan BX, Wang P, Landeck L, Chen JQ, Li W, Cai SQ, Zheng M, Man XY. PLoS One. 2017 Dec 22;12(12):e0190051. doi: 10.1371/journal.pone.0190051. eCollection 2017. Rottlerin as a therapeutic approach?in psoriasis: Evidence from in vitro and in v...||PubMed ID 29272319|
|Psoriasis 1'reduces psoriasis%u2011like skin inflammation by inhibiting the VDR%u2011mediated nuclear NF%u2011%u03BAB and STAT signaling pathways||PubMed ID 30015892|
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