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Product Info Summary
| SKU: | M02435-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-IL3 Mouse Monoclonal Antibody [Clone ID: OTI3B11]
SKU/Catalog Number
M02435-1
Size
100 µl
Description
Boster Bio IL-3 mouse monoclonal antibody, clone OTI3B11 (formerly 3B11). Catalog# M02435-1. Tested in IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-IL3 Mouse Monoclonal Antibody [Clone ID: OTI3B11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02435-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3B11
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 20-152 of human IL-3 (NP_000579) produced in E.coli.
Reactive Species
M02435-1 is reactive to IL3 in Human
Calculated molecular weight
17.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02435-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500
IHC 1:50
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-3.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IL-3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunofluorescent staining of A549 cells using anti-IL-3 mouse monoclonal antibody (M02435-1).
Specific Publications For Anti-IL3 Mouse Monoclonal Antibody [Clone ID: OTI3B11] (M02435-1)
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